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Mineralized keratin biomimetic material and preparation method thereof

A technology of bionic materials and keratin, which is applied in the field of biomedical materials, can solve the problems of unsatisfactory and stable promotion of DPSCs proliferation, and achieve the effect of promoting growth

Active Publication Date: 2020-02-14
FOSHAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, mineralization cannot be produced in a vacuum, and biomacromolecules are needed as scaffolds and media, and the ideal mineralized microenvironment has not been obtained at present, which can effectively and stably promote the proliferation of DPSCs and differentiate into odontocytes

Method used

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  • Mineralized keratin biomimetic material and preparation method thereof
  • Mineralized keratin biomimetic material and preparation method thereof
  • Mineralized keratin biomimetic material and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1: Construction of mineralized keratin biomimetic material

[0034] A preparation method of mineralized keratin biomimetic material, which comprises the following steps:

[0035] 1) Take nano-scale keratin powder and add it into a sterile simulated body fluid environment at a concentration of 100mg / ml, let it stand for mineralization treatment for 6 weeks, stir gently every 12 hours to disperse, and take a sample every other week as a sample. Put the mineralized keratin in a 1.5ml EP tube, centrifuge at 10,000rpm at room temperature for 15min, discard the supernatant, collect the precipitate, resuspend with deionized sterile water and wash 3 times, vacuum freeze-dry, and grind to a particle size of 50nm powder to obtain mineralized keratin particles that have been mineralized for 1 to 6 weeks and used for subsequent physical and chemical characterization experiments;

[0036] 2) The mineralized keratin particles obtained in step 1) that have been mineralized fo...

Embodiment 2

[0037] Example 2: Construction of mineralized keratin biomimetic material

[0038] A preparation method of mineralized keratin biomimetic material, which comprises the following steps:

[0039] 1) Take nano-scale keratin powder and add it into a sterile simulated body fluid environment at a concentration of 0.5 mg / ml, let it stand for mineralization treatment for 6 weeks, stir gently every 12 hours to disperse, take a sample when the mineralization is completed, and place the mineralized keratin In a 1.5ml EP tube, centrifuge at 15000rpm at room temperature for 1min, discard the supernatant, collect the precipitate, resuspend with deionized sterile water and wash 3 times, vacuum freeze-dry, and grind into a powder with a particle size of 20nm to obtain ore keratin particles;

[0040] 2) The mineralized keratin particles obtained in step 1) were resuspended at a concentration of 500mg / ml and poured into 24-well cell culture plates, and after they settled at the bottom of the 2...

Embodiment 3

[0041] Example 3: Construction of mineralized keratin biomimetic material

[0042] A preparation method of mineralized keratin biomimetic material, which comprises the following steps:

[0043] 1) Take nano-scale keratin powder and add it into a sterile simulated body fluid environment at a concentration of 500mg / ml, let it stand for mineralization treatment for 6 weeks, stir gently every 12 hours to disperse, take a sample when the mineralization is completed, and place the mineralized keratin in In a 1.5ml EP tube, centrifuge at 1000rpm for 30min at room temperature, discard the supernatant, collect the precipitate, resuspend with deionized sterile water and wash 3 times, vacuum freeze-dry, grind into a powder with a particle size of 80nm, and obtain mineralized Keratin particles;

[0044] 2) The mineralized keratin particles obtained in step 1) were resuspended at a concentration of 0.1 mg / ml and injected into a 24-well cell culture plate. After they settled at the bottom ...

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Abstract

The invention discloses a preparation method of a mineralized keratin biomimetic material, which comprises the following steps: 1) adding a nanoscale keratin powder agent into a sterile simulated bodyfluid environment at the concentration of 0.1-500mg / ml, performing standing for mineralization treatment, collecting, carrying out vacuum freeze drying, and grinding into powder to obtain mineralizedkeratin particles; and 2) resuspending the mineralized keratin particles at a concentration of 0.1-500 mg / ml, injecting the resuspended mineralized keratin particles into a template, and drying the template in an oven to obtain the membranous mineralized keratin biomimetic material. According to the invention, a biomimetic synthesis method is adopted to induce self-mineralization of keratin, andthe mineralized keratin biomimetic material is constructed on the premise of not introducing other protein factors or other biomacromolecules. The practice shows that the biomimetic material can provide an ideal nano mineralized microenvironment for growth and proliferation of the DPSCs so as to promote the growth of the DPSCs, so that an important reference value is provided for feasibility of dental pulp tissue regeneration and repair.

Description

technical field [0001] The invention relates to biomedical materials, in particular to a mineralized keratin bionic material. Background technique [0002] Pulp inflammation, pulp necrosis and various pulp diseases are common and high-incidence oral diseases. The pulp tissue necrosis and severe inflammatory reaction accompanied by the disease will cause severe pain and affect the normal diet and life of patients. cause adverse effects. Currently, root canal filling is the main treatment for this type of disease. Because the disease has caused damage to the tissues and cells inside the root canal of the tooth, the treatment idea is to kill or remove the normal and diseased tissues in the pulp cavity, and then use Materials or medicines are used to fill root canals to relieve pain, reduce inflammation and prevent the spread of disease. Root canal filling materials can be divided into three types according to their physical properties: solid, liquid, and paste. Among them, so...

Claims

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Application Information

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IPC IPC(8): A61L27/22A61L27/50
CPCA61L27/227A61L27/50A61L2430/12C08L89/00
Inventor 陈吾雅黄达鸿李霞
Owner FOSHAN UNIVERSITY
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