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Biomarker for detecting colorectal cancer

A biomarker and colorectal cancer technology, applied in the field of protein or peptide biomarkers, can solve the problems of accuracy and specificity, difficulty in simultaneous evaluation, etc., and achieve the effect of high specificity detection and high sensitivity detection

Pending Publication Date: 2020-02-14
NAT INST OF BIOMEDICAL INNOVATION HEALTH & NUTRITION +1
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

These methods greatly depend on the quality of antibodies, so there are problems with precision, especially specificity, and it is difficult to simultaneously evaluate multiple marker candidates

Method used

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  • Biomarker for detecting colorectal cancer
  • Biomarker for detecting colorectal cancer
  • Biomarker for detecting colorectal cancer

Examples

Experimental program
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Embodiment

[0168]The present invention is specifically illustrated by the following examples, but the present invention is not limited by these examples.

[0169] method

[0170] Colorectal cancer patients and healthy normal human serum and colorectal cancer cultured cells

[0171] Serum was collected from 51 patients with colorectal cancer and 26 healthy people (collected at Chiba University Medical Center). Serum samples from each specimen were stored at -80°C until analysis.

[0172] Four colorectal cancer cell lines HCT116 (ATCC; CCL-247), DLD-1 (ATCC; CCL-221), SW480 (ATCC; CCL-228) and SW620 (ATCC; CCL-227) were treated with 10% bovine serum ( FBS) and antibiotic RPMI-1640 medium (Gibco Laboratories).

[0173] Incubate each cell in 5% CO 2 The incubator was maintained at 37°C until the cells proliferated to a sub-confluent state, and each cell proliferated to a sub-confluent state. Then, these cultured cells were washed with FBS-free medium, and fresh FBS-free medium was added...

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Abstract

Provided is a biomarker for detecting colorectal cancer at an earlier stage. A colorectal cancer biomarker for detecting colorectal cancer includes at least one protein selected from 22 proteins, i.e., proteins 1 to 22, or at least one peptide selected from partial peptides of proteins 1 to 22: 1) annexin A11; 2) annexin A3; 3) annexin A4; 4) tenascin-N; 5) transferrin receptor protein 1; 6) glucose transporter 1; 7) complement component C9; 8) CD88 antigen; 9) 78-kDa glucose-regulated protein; 10) alpha-1-acid glycoprotein; 11) matrix metalloprotease 9; 12) angiopoietin-1; 13) CD67 antigen; 14) mucin-5B; 15) adapter protein GRB2; 16) annexin A5; 17) olfactomedin-4; 18) neutral amino acid transporter B(0); 19) tripeptidyl peptidase 1; 20) heat shock-related 70-kDa protein 2; 21) proteasomesubunit alpha type-5; and 22) neutrophil gelatinase-associated lipocalin.

Description

technical field [0001] The present invention relates to novel protein or peptide biomarkers that can be used to detect colorectal cancer. Background technique [0002] Colorectal cancer (CRC) is one of the most frequent cancers in the world, and the development of effective biomarkers for CRC is indispensable for improving the survival rate of humans. However, currently used diagnostic methods for colorectal cancer are all diagnostic methods with very low accuracy such as fecal occult blood and CEA, and new biomarkers that can diagnose colorectal cancer at an early stage with high accuracy have not been found. Blood biomarkers are urgently needed . [0003] So far, although multiple cancer-related factors and biomarker candidates for cancer have been discovered through large-scale omics studies, there are no blood biomarkers that have reached clinical use. As proof of this point, there are very few biomarkers recognized by the US Food and Drug Administration (FDA) in recen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68C07K14/47C07K14/705C12N9/64G01N33/53G01N33/574
CPCC12N9/64G01N33/57419G01N33/57473G01N33/6848G01N33/57488G01N2333/70596G01N2333/96494G01N2333/4725C07K14/705C07K14/47C07K14/472G01N33/57484G01N33/6893G01N2333/4609G01N2333/705
Inventor 朝长毅白水崇
Owner NAT INST OF BIOMEDICAL INNOVATION HEALTH & NUTRITION
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