A kind of anti-ultraviolet and high-temperature-resistant high-virulence Ijuvium javanica mutagenic strain ijuv-6 and its application

An IJUV-6, anti-ultraviolet technology, applied in the fields of application, fungi, and microbial-based methods, can solve the problem of normal growth pest infestation, unstable control effect of rice pests, and poor control effect of I. japonicus on rice pests. Stability and other issues

Active Publication Date: 2022-04-08
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to existing research reports (such as CN201711431519.5), I. javanica can effectively control rice pests, but environmental conditions such as ultraviolet rays and high temperatures in paddy fields seriously affect the normal growth of I. javanica and its infestation of pests, resulting in The control effect of I. javanica on rice pests is not stable
Therefore, the problem of unstable control effect of I. javanica on rice pests needs to be solved urgently.

Method used

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  • A kind of anti-ultraviolet and high-temperature-resistant high-virulence Ijuvium javanica mutagenic strain ijuv-6 and its application
  • A kind of anti-ultraviolet and high-temperature-resistant high-virulence Ijuvium javanica mutagenic strain ijuv-6 and its application
  • A kind of anti-ultraviolet and high-temperature-resistant high-virulence Ijuvium javanica mutagenic strain ijuv-6 and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 Isolation and Identification of the Mutagenic Strain IJUV-6 of I. Javanese

[0032] 1. Source of materials

[0033] The mutagenic strain of I. javanica was derived from the wild strain of I. javanica IJNL-N8. The wild strain of I. javanica IJNL-N8 was collected from the brown planthopper [Nilaparvata lugens(Stal)] worm corpse;

[0034] Potato Dextrose Agar medium (Potato Dextrose Agar, PDA medium): 200g of potatoes, 20g of glucose, 20g of agar, add water and boil to make the total volume 1000mL, and sterilize by autoclaving (121°C, 30min);

[0035] Chapek's medium (Czapek): 30.0 g of sucrose, ammonium nitrate (NH 4 NO 3 )3.0g, dipotassium hydrogen phosphate (K 2 HPO 4 ) 1.0g, magnesium sulfate (MgSO 4 .7H 2 O) 0.5g, potassium chloride (KCl) 0.5g, ferrous sulfate (FeSO 4 ) 0.01g, pour it into a beaker and add water to heat and dissolve to make the total volume 1000mL, then sterilize by autoclave (121°C, 20min);

[0036] Culture conditions: Culture in a...

Embodiment 2

[0106] Example 2 Determination of the pathogenicity of I. javanica mutagenic strain IJUV-6 to white-backed planthopper

[0107] 1. Experimental method

[0108] Insects and host plants for testing: Inoculate the white-backed planthopper adults raised on rice plants in the net room to insect-free rice plants, place them in insect cages (60×60×80 cm), and wait for the white-backed planthoppers on rice plants When the back planthopper develops to the 4th instar nymph, it is ready for use.

[0109] Rice is a conventional cultivar, and potted seedlings with a height of 30 cm after tillering are used in experiments.

[0110] Treatment of the tested strains: inoculate the wild strain (WT) and the IJUV-6 mutagenized strain IJUV-6 on PDA medium, and cultivate them in an incubator (14L: 10D) at 26±1°C for 10 days. Add 0.1% Tween-80 sterile water to collect the spores, break up the spore suspension on a magnetic stirrer, filter the impurities with medical gauze to obtain the spore suspe...

Embodiment 3

[0118] Example 3 Determination of the pathogenicity of I. javanica mutagenic strain IJUV-6 to the electric leafhopper

[0119] 1. Experimental method

[0120] Insects and host plants for testing: Inoculate the adult leafhoppers raised on rice in the net room to insect-free rice plants, place them in insect cages (60×60×80 cm), and wait for the electric leafhoppers on the rice plants to grow. Cicada is standby when developing to 3,4,5 instar nymphs.

[0121] Rice is a conventional cultivar, and potted seedlings with a height of 30 cm after tillering are used in experiments.

[0122]Treatment of tested strains: Inoculate the IJUV-6 mutagenic strain (IJUV-6) in PDA medium, cultivate it in a thermostat (14L: 10D) at 26±1°C for 10 days, and add 0.1% sporulation after sporulation. Spores were collected in sterile water at temperature -80°C, and the spore suspension was dispersed on a magnetic stirrer, then filtered with medical gauze to remove impurities, and the spore suspension ...

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Abstract

The invention discloses an ultraviolet-resistant and high-temperature-resistant highly virulent IJUV-6 mutant strain IJUV-6 and an application thereof. The present invention firstly provides an anti-ultraviolet and high-temperature-resistant highly virulent IJUV-6 mutagenic strain IJUV-6, which was preserved in the China Center for Type Culture Collection on October 10, 2019, and its preservation number is CCTCC NO: M 2019800. The IJUV‑6 mutant strain of I. javanica has the characteristics of strong tolerance to ultraviolet light and high temperature, fast colony growth rate, and high spore production. It has strong infection and killing effects on rice planthoppers and rice leafhoppers. Insect effect; In addition, the strain is a mutagenic strain of the wild strain IJNL‑N8 of the native I. japonicum in my country, which is not introduced from abroad, and can adapt to the natural environment such as high temperature and strong ultraviolet radiation in rice fields in southern China; therefore, the Java IJUV‑6, a mutant strain of Isystridium clavulata, can be used as an alternative control drug for drug-resistant pests, and has broad application prospects in the preparation of biological agents for the control of rice planthoppers and rice leafhoppers.

Description

technical field [0001] The invention belongs to the technical field of biological control. More specifically, it relates to a highly virulent I. javanica mutagen strain IJUV-6 resistant to ultraviolet rays and high temperature and its application. Background technique [0002] Both the white-backed planthopper and the electric leafhopper are important pests of rice. They are widely distributed in rice-growing areas in my country, and serious occurrences can lead to large-scale rice yield reduction. The adult white-backed planthopper lays eggs on both sides of the midrib of the leaf sheath and in the midrib tissue of the leaf, and the number of grains per egg is 2 to 31, with an average of 7.3; the nymphs of the white-backed planthopper inhabit the stems and leaf sheaths of rice and do damage , cause discoloration of rice stems and leaves, and when serious damage occurs, partial or large areas of rice fields will die, showing the appearance of yellow pond damage. The adult ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12N13/00A01N63/30A01P7/04C12R1/645
CPCC12N13/00C12R2001/645C12N1/145
Inventor 黄振
Owner SOUTH CHINA AGRI UNIV
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