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Application of genkwanin in preparation of drug for resisting Listeria monocytogenes infection

A technology of listeria and genkwain, applied in the field of medicine

Inactive Publication Date: 2020-02-21
JILIN UNIV
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  • Application Information

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Problems solved by technology

[0002] Genkwanin (Genkwanin) is a class of flavonoids extracted from the plant Genkwa. It has been reported that Genkwanin has various biological activities such as anti-inflammation and anti-oxidation, but it has not been used to treat Listeria monocytogenes. Reports on bacillosis

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  • Application of genkwanin in preparation of drug for resisting Listeria monocytogenes infection
  • Application of genkwanin in preparation of drug for resisting Listeria monocytogenes infection
  • Application of genkwanin in preparation of drug for resisting Listeria monocytogenes infection

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Embodiment Construction

[0011] Through the rabbit erythrocyte hemolysis test, the protective effect test on J774 cells and the mouse infection experiment therapeutic experiment, it is proved that genkwain can effectively inhibit the pore-forming activity of Listeria monocytogenes hemolysin and the pathogenicity of Listeria monocytogenes force inhibition.

[0012] 1. Hemolysis test

[0013] The purified listeria monocytolysin was added to a 96-well plate for serial double dilution, and 10 7 Rabbit red blood cells were incubated at 37°C for 30 minutes and then centrifuged. The highest dilution factor that lysed red blood cells was regarded as the hemolysis titre. Table 1 shows the hemolytic titer of listeriatolysin without adding genkwain and adding different concentrations of genkwain.

[0014] Table 1. Inhibitory effect of Genkwain on the pore-forming activity of Listeria monocytolysin

[0015]

[0016] 2. The protective effect of genkwain on J774 cells

[0017] J774 cells were plated in 96-w...

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Abstract

The invention provides an application of genkwanin in preparation of a drug for resisting Listeria monocytogenes infection. Rabbit erythrocyte hemolysis test, J774 cell protection effect test and mouse infection experiment therapeutics test prove that the genkwanin can effectively inhibit the pore-forming activity of Listeria monocytogenes hemolysin at an extremely low concentration, can inhibit the cytotoxic effect of Listeria monocytogenes on J774 cell mediation, and has a good treatment effect on mouse infection caused by the Listeria monocytogenes. According to the invention, Listeria monocytogenes infection is treated by the genkwanin. Compared with the traditional antibiotic treatment, the genkwanin is not easy to induce the generation of drug resistance, has lower selection pressureon bacteria, has no obvious toxic effect in the test, and has higher cure rate. Therefore, the genkwanin can be used for new drug development and has important significance for drug target confirmation.

Description

technical field [0001] The invention relates to the application of genkwain in the preparation of drugs for resisting Listeria monocytogenes infection, and belongs to the field of medicine. Background technique [0002] Genkwanin (Genkwanin) is a class of flavonoids extracted from the plant Genkwan. It has been reported that Genkwanin has various biological activities such as anti-inflammation and anti-oxidation, but it has not been used to treat Listeria monocytogenes. Reports on bacillosis. [0003] Listeria monocytogenes is an important facultative anaerobic, zoonotic intracellular pathogen and the causative bacterium of listeriosis. It is widely distributed in the environment, resistant to high temperature, high salt and high pH value environment, can proliferate in water, oil, fertilizer, animal feed, dairy products, refrigerators, and infect humans and livestock through fecal-oral transmission, drinking water and other ways. As an opportunistic pathogen, Listeria mon...

Claims

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Application Information

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IPC IPC(8): A61K31/352A61K36/83A61P31/04
CPCA61K31/352A61K36/83A61P31/04
Inventor 冯海华徐蕾王建锋卢葛锦邓旭明
Owner JILIN UNIV
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