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Preparation method of metarrhizium anisopliae high-spore powder

A technology for Metarhizium anisopliae and sporopollen is applied in the field of preparation of Metarhizium anisopliae high sporopollen, which can solve the problems of polluted environment and agricultural, forestry and animal husbandry products, difficult separation, destruction of natural ecosystems, etc., so as to ensure the growth environment and reduce the cultivation cost. , the effect of easy collection

Pending Publication Date: 2020-02-21
琼台师范学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Since the middle of the 20th century, due to the unlimited use of chemical pesticides, the environment and agricultural, forestry and animal husbandry products have been seriously polluted, endangering the health of humans and animals, and at the same time destroying the natural ecosystem
[0003] At present, the conidia of Metarhizium anisopliae at home and abroad are mainly obtained by liquid-solid two-phase fermentation, that is, liquid seeds are inoculated into conventional rice and other nutrient substrates for solid dosage form solid fermentation, but the use of rice and other substrates is relatively expensive, and at the same time Difficult to separate later

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A preparation method for metarhizium anisopliae high sporopollen, comprising the following steps:

[0024] S1. Making a slant culture medium for Metarhizium anisopliae strains in a test tube, and using the slant medium to subculture and activate the strains of Metarhizium anisopliae, and the culture temperature for the strains of Metarhizium anisopliae is maintained at 23-28°C;

[0025] S2. When the surface of the slant medium in the test tube is covered with spores, take part of the spores and bacterial lawn from the slant medium, insert the spores and bacterial lawn into the liquid medium, and the liquid medium is sterilized by steam and kept at room temperature;

[0026] S3. Cultivate the liquid culture medium containing spores and bacterial lawns in a constant temperature shaking box at a constant temperature of 26° C., and the constant temperature cultivation time is 2-3 days, until viscous mycelium appears in the liquid culture medium;

[0027] S4. Mix the liquid ...

Embodiment 2

[0033] A preparation method for metarhizium anisopliae high sporopollen, comprising the following steps:

[0034] S1. Making a slant culture medium for Metarhizium anisopliae strains in a test tube, and using the slant medium to subculture and activate the strains of Metarhizium anisopliae, and the culture temperature for the strains of Metarhizium anisopliae is maintained at 23-28°C;

[0035] S2. When the surface of the slant medium in the test tube is covered with spores, take part of the spores and bacterial lawn from the slant medium, insert the spores and bacterial lawn into the liquid medium, and the liquid medium is sterilized by steam and kept at room temperature;

[0036] S3. Cultivate the liquid culture medium containing spores and bacterial lawns in a constant temperature shaking box at a constant temperature of 26° C., and the constant temperature cultivation time is 2-3 days, until viscous mycelium appears in the liquid culture medium;

[0037] S4. Mix the liquid ...

Embodiment 3

[0043] A preparation method for metarhizium anisopliae high sporopollen, comprising the following steps:

[0044] S1. Making a slant culture medium for Metarhizium anisopliae strains in a test tube, and using the slant medium to subculture and activate the strains of Metarhizium anisopliae, and the culture temperature for the strains of Metarhizium anisopliae is maintained at 23-28°C;

[0045] S2. When the surface of the slant medium in the test tube is covered with spores, take part of the spores and bacterial lawn from the slant medium, insert the spores and bacterial lawn into the liquid medium, and the liquid medium is sterilized by steam and kept at room temperature;

[0046] S3. Cultivate the liquid culture medium containing spores and bacterial lawns in a constant temperature shaking box at a constant temperature of 26° C., and the constant temperature cultivation time is 2-3 days, until viscous mycelium appears in the liquid culture medium;

[0047] S4. Mix the liquid ...

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PUM

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Abstract

The invention discloses a preparation method of metarrhizium anisopliae high-spore powder. The preparation method comprises the following steps of preparing metarrhizium anisopliae strain slant culture mediums in a test tube, and performing subculturing and activation on metarrhizium anisopliae strains through the slant culture mediums; when spores cover the surfaces of the slant culture mediums in the test tube, taking part of spores and bacterial lawns from the slant culture mediums, and inoculating liquid culture mediums with the spores and the bacterial lawns; performing constant-temperature culturing on the liquid culture mediums containing the spores and the bacterial lawns in a constant-temperature vibration box until viscous mycelia appear in the liquid culture mediums; and mixingthe liquid culture mediums in which the viscous mycelia appear, with a nutrient solution to obtain a mixed metarrhizium anisopliae culture solution, uniformly spraying the culture solution on a tiledcloth block, performing steam high-temperature sterilization on the cloth block, and placing the cloth block after the steam high-temperature sterilization in a fermentation vessel for fermentation. According to the preparation method of the metarrhizium anisopliae high-spore powder, the cloth block is used as a culture carrier, so that the culture cost can be reduced, and besides, later-stage collection of spores is convenient; and oxytetracycline is added, so that growing environment of the metarrhizium anisopliae can be guaranteed.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation, and in particular relates to a method for preparing high sporopollen of Metarhizium anisopliae. Background technique [0002] Since the mid-20th century, due to the unlimited use of chemical pesticides, the environment and agricultural, forestry and animal husbandry products have been seriously polluted, endangering the health of humans and animals, and at the same time destroying the natural ecosystem. Biological control has been paid more and more attention by people, and the use of entomopathogenic microorganisms to control pests is one of the important means of biological control. Fungal insecticides have the characteristics of good diffusion effect, long persistence, unique body wall invasion, no resistance to pests and easy mass production, making fungal insecticides widely used in forest pests and soil pests. It has obvious advantages in the biological control of piercing-...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14
Inventor 许天委李国寅丁兆建郝慧华吴小霞
Owner 琼台师范学院