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Biological inoculant having enhanced fertilizing and fungicidal activity

An inoculant and biological technology, applied in the direction of biocides, microorganisms, fungi, etc., can solve problems such as the combination of beneficial bacteria and biological antifungal agents that have not been considered

Inactive Publication Date: 2020-02-21
YPF TECHA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0016] To the best of the inventors' knowledge, no combination of beneficial bacteria and biological antifungal agents has been considered for successful application in agricultural fields to date

Method used

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  • Biological inoculant having enhanced fertilizing and fungicidal activity
  • Biological inoculant having enhanced fertilizing and fungicidal activity
  • Biological inoculant having enhanced fertilizing and fungicidal activity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] [Example 1: Determination of Antagonist Ability of Isolated Trichoderma (Trichoderma) Strains]

[0058] A total of 60 Trichoderma isolates were obtained from roots of crop plants as well as soil samples as described previously. These samples were identified by morphology, colony pigmentation and identification of the unique structure of the genus by microscopy techniques.

[0059] To determine the antagonistic activity of soil isolates, resistance tests were carried out with a series of phytopathogenic fungi, including those responsible for the most famous diseases in plant crops: Fusarium sp., Colletotrichum sp., Cercospora sp., Sclerotinia sp. and Rhizoctonia sp.

[0060] These fungal pathogens belong to many agronomically relevant crops such as soybean, wheat, corn, sunflower, cotton, sorghum, alfalfa, flax, canola, chickpea, rice, potato, onion, yerba mate (Ilexparaguariensis) , Tea And Vine.

[0061] During several growth rounds in the presence of the aforementi...

Embodiment 2

[0066] [Example 2: Characterization of the interaction of isolated Trichoderma strains with soybean]

[0067] The effect of three isolated Trichoderma strains on the growth of soybean plants under greenhouse conditions was determined. To achieve this, the most effective isolates for controlling the proliferation of pathogenic fungi were selected and the concentration of conidia in inoculated plants was optimized (Samuels & Hebbar, 2015). Build biological inoculants to produce in as little as 10 6 It is still effective at the concentration of spores / ml.

[0068] Subsequent measurements demonstrated that the three selected Trichoderma harzianum strains colonized the roots of soybean plants in the presence of B. japonicum without reducing their natural nodulation capacity. Furthermore, treatment of soybean seeds with these T. harzianum strains even resulted in an increase in the number of nodules per root weight, with Trch3 showing a greater effect and also maintaining the prop...

Embodiment 3

[0072] [Example 3: Trichoderma (Trichoderma)-Bradyrhizobium (Bradyrhizobium) interaction assay]

[0073] Crops were established and grown to evaluate the growth capabilities of two microorganisms: Bradyrhizobium japonicum and Trichoderma.

[0074] Soybean plants were also assessed for nodulation ability in the presence of Trichoderma compared to control plants. These trials were performed under controlled field crop conditions and allowed to evaluate the effect of fungal protection treatments on seeds, nodulation capacity, different growth parameters and final soybean yield.

[0075] The treatment is applied to premium soybeans. The experiment was carried out in the municipality of Pergamino, located in the north of the province of Buenos Aires (33°57'51.87"S 60°34'36.89"W), on soils of the Pergamino series, typical Argiudol, mixed family, Franca Soil texture, thermal, class I-2, IP=85. Sowing was carried out with soybean variety DM 4615STS with a row spacing of 0.40 m. Co...

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PUM

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Abstract

A biological inoculant having both fertilizing and fungicidal activity is disclosed. More particularly, a bio-inoculant having the combined effects, comprisingBradyrhizobium japonicumand specific isolates from theTrichodermagenus is disclosed. The bio-inoculant is applied to soybean crops for preventing fungi borne diseases. More particularly, the bio-inoculant of the invention is useful for protecting soybean crops against infection byFusarium sp., Colletotrichum sp., Cercospora sp., Sclerotinia sp.andRhizoctonia sp.

Description

[0001] 【Field of Invention】 [0002] The present invention generally relates to the field of agricultural technology for improving the performance of industrial crops. In particular, the present invention relates to biological inoculants having fertilizing and fungicidal activity which enhance the growth and yield of crop plants. More specifically, the present invention relates to a biological inoculant comprising Bradyrhizobium japonicum and a specific isolate from the genus Trichoderma with said combined effect. In one embodiment of the invention, a biological inoculant is applied to soybean crops to prevent fungal borne diseases. More specifically, the biological inoculants of the present invention can be used to protect soybean crops from Fusarium sp., Colletotrichum sp., Cercospora sp., Sclerotinia sp. sp.) and Rhizoctonia sp. 【Background technique】 [0003] Millions of hectares of soybeans are sown every year around the world. Different seeding areas span multiple en...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/14A01C1/00C12R1/01C12R1/885
CPCC12N1/20C12N1/14A01C1/00C05F11/08A01N63/38C12R2001/885C12N1/145A01N63/20A01N63/30C05G3/60A01N63/00
Inventor W·A·瓦格斯S·雷诺索M·C·奥尔西尼S·A·特乔E·阿布拉莫维奇
Owner YPF TECHA
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