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Escherichia coli O157 immunizing magnetic bead lotion

An immunomagnetic bead, O157 technology, applied in the field of immunity, can solve the problems of reducing the detection rate of target bacteria, reducing the cleaning effect, and increasing other pathogenic bacteria, so as to meet the needs of modern industrial production, improve the efficacy of immune cleaning, and improve the detection rate. Effect

Pending Publication Date: 2020-02-21
GUANGDONG HUANKAI BIOLOGICAL SCI & TECH CO LTD +2
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

However, the conventional immunomagnetic bead washing solution did not play a very good role
Especially for the enrichment of minced meat samples, a large number of immunomagnetic beads are lost, thereby reducing the detection rate of target bacteria
Moreover, the cleaning effect is reduced, and other pathogenic bacteria are added, so that the cleaning process cannot meet the experimental requirements.

Method used

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  • Escherichia coli O157 immunizing magnetic bead lotion
  • Escherichia coli O157 immunizing magnetic bead lotion
  • Escherichia coli O157 immunizing magnetic bead lotion

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1～8

[0040] The Escherichia coli O157 immunomagnetic bead washing solution of Examples 1-8 was weighed according to the formula 1-8 in Table 1, and the corresponding solution was prepared using PBS buffer (0.1M, PH7.4); mixed and stirred Evenly, so that the pH of the washing solution is 7.0-7.5, the Escherichia coli O157 immune magnetic bead washing solution is obtained.

[0041] The conventional lotion formulation is a PBS (0.1M, PH7.4) solution containing 0.5g / L Tween-20.

[0042] Table 1 Different formulation components of immunomagnetic bead washing solution

[0043]

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Abstract

The invention discloses an Escherichia coli O157 immunizing magnetic bead lotion. The Escherichia coli O157 immunizing magnetic bead lotion is prepared through mixing a surfactant, proteins, water-soluble saccharides, inorganic salts and a bacteriostatic agent at a specific ratio, wherein a buffer solution serves as a solvent. Compared with the conventional magnetic bead lotions, the Escherichia coli O157 immunizing magnetic bead lotion has the advantages that besides the cleaning effect is improved, bacteria are further specifically and continuously increased, and the detectable rate of Escherichia coli O157 is increased. Furthermore, the interference caused by food matrixes is effectively reduced, the sensitivity of the immunizing magnetic bead lotion is improved, and defects of the conventional magnetic bead lotions are overcome.

Description

technical field [0001] The invention belongs to the technical field of immunization, in particular to an Escherichia coli O157 immune magnetic bead washing solution. Background technique [0002] Escherichia coli O157:H7 is a more prominent serotype strain in Enterohemorrhagic Escherichia coli (EHEC), and the infection dose of Escherichia coli O157:H7 is extremely small (only 10 bacteria). After infection, patients may develop symptoms such as diarrhea, and further develop into hemorrhagic colitis. The disease progresses extremely quickly and has a high fatality rate, causing serious harm to society and public health. [0003] In food-borne EHEC infection, meat and its products, milk, fruits and their products can be contaminated. It is difficult to detect this pathogen in the conventional E. coli detection. In order to improve the detection rate, molecular detection products are constantly being produced. With the emergence of the detection method, the sensitivity has been...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/10
CPCC12Q1/10
Inventor 曲晓莹蔡芷荷卢勉飞徐环吴清平巩路陈鲁
Owner GUANGDONG HUANKAI BIOLOGICAL SCI & TECH CO LTD
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