A Fluorescent Spectrum Detection System for Microorganisms

Abstract

The invention discloses a fluorescent spectrum detection system for microorganisms, which includes a laser light source emitting laser light to a microbial sample to be tested carried on a light-transmitting platform to excite a fluorescent dye to emit fluorescence; the light-transmitting platform is arranged on the focal plane of a microlens group ; The microlens group amplifies the fluorescent light transmitted by the light-transmitting platform to form parallel light; the filter element filters the parallel light; the first condenser lens group condenses the parallel light to form a fluorescent image, which is divided by the integral field of view unit into several image units, and the spectrometer is used to disperse the several image units; the detector detects the obtained spectral distribution information. This application does not need to scan the fluorescent light in the field of view of the microlens group position by position, and realizes the synchronous dispersion of the fluorescent light in the field of view of the microlens group, which improves the observation efficiency of the fluorescence detection of microbial samples, and is beneficial to the Capturing the instantaneous changes of cells or organelles in microorganisms improves the accuracy of microbial research.

Description

technical field [0001] The invention relates to the technical field of microorganism observation, in particular to a microorganism fluorescence spectrum detection system. Background technique [0002] Fluorescence imaging is an indispensable tool for biological research, especially in cell research. By staining cells or organelles with various fluorophores and zooming in on images under a microscope, a large number of color-coding processes can be quantitatively characterized, enabling the study of cellular gene expression based on chromosome dynamics. Fluorophores have greatly improved cellular research by bringing high quantum yield fluorescent dyes and multiplex staining methods to the field. This enables researchers to study several organelles and their interactions simultaneously in the same FOV (field of view) with high contrast. [0003] Hyperspectral fluorescence microscopy with high temporal resolution is gaining in importance in biological microscopy as it can be...

AI Technical Summary

Problems solved by technology

But most currently available hyperspectral fluorescence microscopes undergo a scanning process, which limits their temporal resolution and, in turn, their potential use in real-time imaging.

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