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Synthesis method of fatty acid

A synthesis method and fatty acid technology, applied in fermentation and other directions, can solve problems such as long reaction time, achieve the effects of mild reaction conditions, shorten hydrolysis time, and avoid high temperature and high pressure

Inactive Publication Date: 2020-02-25
SOUTH CHINA UNIV OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Li Lei (CN109957459 A) used G 50 Auxiliary TL hydrolyzes castor oil, the hydrolysis rate of glyceride can reach 94.77%, but the reaction time is as long as 24h

Method used

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  • Synthesis method of fatty acid

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The mass ratio (1:0.5) of soybean oil and water (phosphate buffer solution with a pH of 7.5) was added into a stoppered Erlenmeyer flask, mixed evenly, and placed on a constant temperature magnetic stirrer with a rotation speed of 500rpm to preheat at 40°C for 10min. Add 400U / g of triglyceride lipase Lipase TL100L (based on the total mass of oil), 100U / g of diglyceride lipase SMG1-F278N (based on the total mass of oil) after the heat is over, and add 50U / g of monoglycerol at the same time For ester lipase GMGL-S147A (based on the total mass of the oil), the reaction temperature was controlled at 40° C., and samples were taken to detect the hydrolyzate at 12 hours. The experimental results are shown in Table 1.

Embodiment 2

[0029] The mass ratio (1:0.5) of soybean oil and water (phosphate buffer solution with a pH of 7.5) was added into a stoppered Erlenmeyer flask, mixed evenly, and placed on a constant temperature magnetic stirrer with a rotation speed of 500rpm to preheat at 40°C for 10min. Add 400U / g of triglyceride lipase Lipase TL100L (based on the total mass of oil), 100U / g diglyceride lipase Lipase PCL-I260R (based on the total mass of oil) after the end of the heat, and add 50U / g of single Glyceride lipase Lipase GMGL-S147A (based on the total mass of oil), the reaction temperature was controlled at 40°C, and samples were taken for 12 hours to detect the hydrolyzate. The experimental results are shown in Table 1.

Embodiment 3

[0031] The mass ratio (1:0.5) of soybean oil and water (phosphate buffer solution with a pH of 7.5) was added into a stoppered Erlenmeyer flask, mixed evenly, and placed on a constant temperature magnetic stirrer with a rotation speed of 500rpm to preheat at 40°C for 10min. Add 400U / g of triglyceride lipase Lipase TL100L (based on the total mass of oil), 100U / g of diglyceride lipase LipaseAOL-V269D (based on the total mass of oil) after the heat is over, and add 50U / g of monoglycerol at the same time For ester lipase Lipase GMGL-S147A (based on the total mass of oil), the reaction temperature was controlled at 40°C, and samples were taken for 12 hours to detect the hydrolyzate. The experimental results are shown in Table 1.

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Abstract

The invention discloses a synthesis method of fatty acid. The synthesis method comprises the following steps: mixing grease, water, triglyceride lipase, diglyceride lipase and monoglyceride lipase, and carrying out a hydrolysis reaction. In the hydrolysis process, a diglyceride lipase mutant and a monoglyceride lipase mutant are used for promoting the triglyceride lipase to hydrolyze grease, so that the rate and the purity of synthesized fatty acid can be remarkably improved. When the three enzymes are used together, the synthesis rate and purity of the fatty acid are higher than those of anyone of the three enzymes used independently, and the hydrolysis rate reaches 95% or above. The synthetic method is rapid, efficient, green and convenient for industrial production.

Description

technical field [0001] The invention relates to a method for synthesizing fatty acids. Background technique [0002] Free fatty acids (FFA) are important raw materials in the oleochemical industry, and they have been widely used in many high-value products such as coatings, surfactants, adhesives, lubricating oils, fatty alcohols, shampoos, and other personal care products with Wide range of industrial uses. [0003] The production methods of fatty acids mainly include saponification, steam cracking, and enzymatic hydrolysis. Obtaining free fatty acids through high-pressure methods is the main technology for industrial fatty acid production. However, this process requires high temperature and high pressure conditions and leads to many side reactions. Not only high energy consumption, but also affect product quality. However, enzymatic hydrolysis has attracted widespread attention because of its mild reaction conditions, unsaturated fatty acids are not easily oxidized, and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64
CPCC12P7/6418
Inventor 王永华刘萱杨博王卫飞蓝东明罗日明
Owner SOUTH CHINA UNIV OF TECH