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Application of NSrp70 gene in preparation of tumor metastasis related pharmaceutical preparations

A technology of pharmaceutical preparations and genes, applied in the field of biomedical engineering, can solve the problems of chemotherapy limitation, reducing the quality of life of patients, and poor prognosis.

Pending Publication Date: 2020-02-25
FUDAN UNIV SHANGHAI CANCER CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Once breast cancer metastasis occurs, it is rarely cured, and the prognosis is poor, which significantly reduces the quality of life of patients and increases the cost of treatment
[0003] At present, the clinical treatment of metastatic breast cancer is divided into two categories: radiotherapy and chemotherapy and targeted therapy; compared with Luminal and HER2+ breast cancer, TNBC cannot use conventional endocrine therapy and targeted therapy because ER and HER2 are not expressed , Chemotherapy is also limited by drug resistance, the prognosis of patients is generally poor, and there is an urgent need for effective treatment options in clinical practice

Method used

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  • Application of NSrp70 gene in preparation of tumor metastasis related pharmaceutical preparations
  • Application of NSrp70 gene in preparation of tumor metastasis related pharmaceutical preparations
  • Application of NSrp70 gene in preparation of tumor metastasis related pharmaceutical preparations

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0247] Example 1NSrp70 is relatively low expressed in breast cancer cells with high metastatic potential

[0248] The nuclear proteins were extracted from the three cell lines MDA-MB-231, MDA-MB-231HM, and MDA-MB-231Bo, and the iTRAQ quantitative proteomics analysis was performed to screen the differentially expressed proteins. The results showed that the expression level of NSrp70 was highly metastatic Significantly down-regulated in the potential cell lines MDA-MB-231HM and MDA-MB-231Bo, attached figure 1 is the iTRAQ mass spectrometry result of NSrp70; attached figure 2 is the result of differential expression of NSrp70 in MDA-MB-231, MDA-MB-231HM and MDA-MB-231Bo cell lines in iTRAQ mass spectrometry;

[0249] Further, the commonly used breast cancer cell line proteins were extracted for Western Blot verification. The results showed that the expression level of NSrp70 in cell lines with strong metastatic ability (such as Hs578T, MDA-MB-231LM2, MDA-MB-231Bo) was significa...

Embodiment 2

[0250] Example 2 NSrp70 inhibits tumor cell migration and invasion in vitro

[0251] In order to further clarify the effect of NSrp70 on metastasis, breast cancer cell lines MDA-MB-231 and BT549 were selected for NSrp70 interference in this example, and MDA-MB-231LM2 and Hs578T were used as NSrp70 overexpressing cell models. The expressions were all successful, with Figure 4 and 5 To detect the effect of NSrp70 interference and overexpression in breast cancer cells by Western blot;

[0252] Further in vitro Transwell chamber migration and invasion assays were performed, and the results showed that in NSrp70-interfered MDA-MB-231 and BT549, NSrp70 downregulation could significantly increase cell migration and invasion abilities, on the contrary, NSrp70 overexpression significantly inhibited MDA-MB- 231LM2 and Hs578T transfer capability, Image 6is the result of Transwell verification of the effect on invasion and migration after interfering with NSrp70 in MDA-MB-231, in whi...

Embodiment 3

[0254] Example 3 NSrp70 inhibits the ability of breast cancer cells to metastasize in vivo

[0255] In order to further clarify the ability of NSrp70 to regulate breast cancer cell metastasis in vivo, MDA-MB-231LM2 was labeled with Luciference fluorescent plasmid, and then MDA-MB-231LM2pCDH and MDA-MB-231LM2NSrp70 were stably transfected into cells. 5 Cells / only were injected into the tail vein of BALB / c nude mice, and live imaging of nude mice was performed 4 weeks later. The results showed that the lung metastases of the mice in the NSrp70 overexpression group were significantly less than those in the control group. It has a significant inhibitory effect on the metastasis of cancer cells in vivo. Figure 12 is the result of in vivo imaging in mice.

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Abstract

The invention belongs to the technical field of biomedical engineering, and relates to a new application of an NSrp70 gene, in particular to an application of the NSrp70 gene in preparation of tumor metastasis related pharmaceutical preparations. The nuclear protein quantitative proteomics analysis is carried out on breast cancer cells; the results show that the nuclear speckle protein NSrp70 shows low expression in breast cancer cells with high metastasis potential, the in-vitro and in-vivo experiments prove that the interference of NSrp70 can significantly promote the metastasis ability of the cells, and overexpression of the gene can significantly inhibit the metastasis ability of the cells; the NSrp70 can inhibit cell EMT by inhibiting a TGF beta signal path, and finally breast cancermetastasis is inhibited; the NSrp70 can be used as a new molecular target and a molecular marker in a breast cancer metastasis process to be applied to preparation of breast cancer metastasis relatedmedicaments, including preparation of a detection kit for detecting cancer metastasis, a medicine for inhibiting cancer metastasis and a medicine for inhibiting a cancer metastasis related signal path. The novel application is helpful for preventing and inhibiting breast cancer metastasis and benefiting cancer patients.

Description

technical field [0001] The invention belongs to the technical field of biomedical engineering, and relates to a new application of NSrp70 gene, in particular to the application of NSrp70 gene in the preparation of pharmaceutical preparations related to tumor metastasis. Background technique [0002] According to public data, breast cancer has become a disease that seriously affects the physical and mental health of women around the world, and its morbidity and mortality are increasing year by year. Studies have shown that breast cancer metastasis is the main cause of breast cancer death. Clinical practice has found that breast cancer can metastasize to multiple organs, including lung, bone, liver, pleura, soft tissue, etc. The median survival time of patients with soft tissue and bone metastasis 22 to 26 months, 10 to 12 months for lung and pleural metastases, and 4 to 6 months for liver and brain metastases. Once breast cancer metastasis occurs, it is rarely cured and has ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886G01N33/68G01N33/574A61K38/17A61P35/04
CPCC12Q1/6886G01N33/68G01N33/57415A61K38/1709A61P35/04C12Q2600/118
Inventor 金伟赵阳孙荷芬
Owner FUDAN UNIV SHANGHAI CANCER CENT
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