Kit for detecting canine pancreatic lipase based on homogeneous chemiluminescence immunoassay method

A homogeneous chemiluminescence, canine pancreatic fat technology, applied in chemiluminescence/bioluminescence, analysis by chemical reaction of materials, analysis of materials, etc., can solve problems such as inconvenience, achieve accurate results, short time-consuming, precise high degree of effect

Active Publication Date: 2020-02-25
南京浦光生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] This shows that the above-mentioned existing canine pancreatic lipase obviously still has inconvenience and defects in the detection method and use, and needs to be further improved urgently.
In order to solve the problems existing in the detection method of canine pancreatic lipase...

Method used

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  • Kit for detecting canine pancreatic lipase based on homogeneous chemiluminescence immunoassay method
  • Kit for detecting canine pancreatic lipase based on homogeneous chemiluminescence immunoassay method
  • Kit for detecting canine pancreatic lipase based on homogeneous chemiluminescence immunoassay method

Examples

Experimental program
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Effect test

Embodiment 1

[0041] Configure detection reagents: Mix DNA1-cPL Antibody 1 Conjugate, DNA2-cPL Antibody 2 Conjugate, AE-modified DNA3, and GO-AOD to make their final concentrations 1nM, 1nM, 0.05μM and 20μg / ml respectively . Mix 20 μL of calibration solution with different concentrations (0, 50, 100, 200, 400, 1000, 2000 ng / ml) with 200 μL of detection solution, place in HSCL-10000 chemiluminescence instrument, and incubate at 37°C for 5 minutes . After incubation, 200 μL of chemiluminescence substrate was added by HSCL-10000 chemiluminescence instrument, and the chemiluminescence signal of the solution was detected by photomultiplier tube (PMT) immediately, and the detection time was 3 s. According to the recorded chemiluminescence value (RLU), the calibration curve of the cPL antigen and the concentration of the cPL antigen in the test sample were obtained. The specific results are shown in Table 1.

Embodiment 2

[0043] Configure detection reagents: Mix DNA1-cPL Antibody 1 Conjugate, DNA2-cPL Antibody 2 Conjugate, AE-modified DNA3, and GO-AOD to make their final concentrations respectively 5nM, 5nM, 0.1μM and 20μg / ml . Mix 20 μL of calibration solution with different concentrations (0, 50, 100, 200, 400, 1000, 2000 ng / ml) with 200 μL of detection solution, place in HSCL-10000 chemiluminescence instrument, and incubate at 37°C for 5 minutes . After incubation, 200 μL of chemiluminescence substrate was added by HSCL-10000 chemiluminescence instrument, and the chemiluminescence signal of the solution was detected by photomultiplier tube (PMT) immediately, and the detection time was 3 s. According to the recorded chemiluminescence value (RLU), the calibration curve of the cPL antigen and the concentration of the cPL antigen in the test sample were obtained. The specific results are shown in Table 1.

Embodiment 3

[0045] Configure detection reagents: mix DNA1-cPL antibody 1 conjugate, DNA2-cPL antibody 2 conjugate, AE-modified DNA3, and GO-AOD, so that their final concentrations are 10 nM, 10 nM, 0.15 μM and 20 μg / ml. Mix 20 μL of calibration solution with different concentrations (0, 50, 100, 200, 400, 1000, 2000 ng / ml) with 200 μL of detection solution, place in HSCL-10000 chemiluminescence instrument, and incubate at 37°C for 5 minutes . After incubation, 200 μL of chemiluminescence substrate was added by HSCL-10000 chemiluminescence instrument, and the chemiluminescence signal of the solution was detected by photomultiplier tube (PMT) immediately, and the detection time was 3 s. According to the recorded chemiluminescence value (RLU), the calibration curve of the cPL antigen and the concentration of the cPL antigen in the test sample were obtained. The specific results are shown in Table 1.

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Abstract

The invention discloses a kit for detecting canine pancreatic lipase based on a homogeneous chemiluminescence immunoassay method. The kit comprises a DNA1-cPL antibody 1 conjugate, a DNA2-cPL antibody2 conjugate, acridinium ester (AE) labeled DNA3 and a graphene oxide (GO) binding antioxidant (AOD). The kit disclosed by the invention is used for detection, and the kit has the advantages of simpleoperation, accurate result, short time consumption, high precision and the realization of quantitative detection.

Description

technical field [0001] The invention relates to the technical field of chemiluminescence immunoassay, in particular to a kit for detecting canine pancreatic lipase based on homogeneous chemiluminescence immunoassay. Background technique [0002] Canine acute pancreatitis is an internal medical disease that is clinically harmful to dogs. Acute pancreatitis has an acute onset, short course of disease, severe clinical symptoms, difficult to cure, and even death. Many acute cases developed into chronic or subdiffuse pancreatitis due to lack of accurate diagnosis and delayed treatment. The recurrence of the disease brings great pain to the suffering dog and also brings a lot of trouble to the dog owner. [0003] At present, in veterinary clinics at home and abroad, the diagnosis of acute pancreatitis mainly relies on the combination of physical examination and laboratory diagnostic indicators. The diagnostic specificity and sensitivity of canine pancreatitis are not strong, and...

Claims

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Application Information

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IPC IPC(8): G01N33/573G01N21/76
CPCG01N21/76G01N33/573G01N2333/92
Inventor 曹丹
Owner 南京浦光生物科技有限公司
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