A kind of autologous umbilical cord mesenchymal stem cell cryopreservation liquid and its preparation and cryopreservation method

Abstract

The invention discloses a cryopreservation liquid for autologous umbilical cord mesenchymal stem cells and its preparation and cryopreservation method. The cryopreservation liquid is composed of liquid A and liquid B used together, and the volume ratio of liquid A to liquid B is 6-9: 1-3; the A solution is calculated in parts by volume, and includes the following components: 65-75 parts of basal medium containing pre-supplements; the ratio of pre-supplements to basal medium is 7-12mg: 0.6-0.9mL; Wherein, the pre-additives are calculated in parts by weight, including: 4-6 parts of Morinda officinalis alcohol extract, 0.13-0.16 parts of type I collagen, 0.05-0.15 parts of sodium hyaluronate and 3-5 parts of lipoic acid; Liquid B is calculated in parts by volume and includes the following components: 15‑25 parts of autologous serum. Using the cryopreservation solution of the present invention can simultaneously obtain a sufficient amount of blood for the separation of autologous serum during the delivery of newborns, thereby effectively avoiding the contamination of serum proteins from heterogeneous allogeneic sources, and at the same time simulating the microenvironment of the extracellular matrix to improve the survival rate of cell resuscitation, Therefore, it is beneficial to its application in the standardized operation of biological sample banks and clinical cell therapy.

Description

technical field [0001] The invention belongs to the technical field of cell preservation, and in particular relates to a cryopreservation liquid for autologous umbilical cord mesenchymal stem cells and a preparation and cryopreservation method thereof. Background technique [0002] Cell cryopreservation is a technique in which cells are placed in a low temperature environment to reduce cell metabolism for long-term storage. Cell cryopreservation is one of the main methods of cell preservation, which plays a role in cell preservation. Using cryopreservation technology to store cells in -196°C liquid nitrogen at low temperature can temporarily remove the cells from the growth state and preserve their cell characteristics, so that the cells can be revived for experiments when needed. Moreover, moderately preserving a certain amount of cells can prevent the cells from being lost due to contamination of the cells being cultured or other accidents, which plays a role in cell pres...

AI Technical Summary

Problems solved by technology

Serum and medium are added to provide cells with suitable osmotic pressure, pH value and nourishing components, but serum proteins from different sources, including heterogeneous or allogeneic sources, increase the chance of cell contamination and do not meet the safety standards of clinical cell therapy preparations

In addition, mesenchymal stem cells are digested into suspended single cells before cryopreservation, and are separated from the cell matrix formed by extracellular secretion in adherent culture, and traditional cryopreservation agents do not contain components that simulate extracellular matrix, so that after cryopreservation and recovery poor cell viability

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