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Process for degrading organic matters in amino acid fermentation waste liquid by utilizing compound microbial inoculant

A technology of compound bacterial agent and fermentation waste liquid, applied in the biological field, can solve the problems of environmental impact, high solid particulate matter, large spray volume, etc.

Active Publication Date: 2020-02-28
HULUNBEIER NORTHEAST FUFENG BIOTECHNOLOGIES CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Fufeng is the first in the industry to adopt "spray adsorption + plasma technology" to remove more than 80% of smoke and odor, and achieve discharge standards. However, this set of equipment uses fermented wastewater for spray cooling in the Venturi spray process. Large, the water temperature is high, and a large amount of water vapor is formed in the second time; although the solid particles in the waste flue gas are effectively removed by the electrostatic precipitator, the solid particles in the exhaust flue gas at the outlet are still high; The exhausted solid particles and the trace organic matter in the concentrated liquid, mainly burnt and burnt, are discharged through the chimney together, which has an impact on the environment

Method used

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  • Process for degrading organic matters in amino acid fermentation waste liquid by utilizing compound microbial inoculant
  • Process for degrading organic matters in amino acid fermentation waste liquid by utilizing compound microbial inoculant
  • Process for degrading organic matters in amino acid fermentation waste liquid by utilizing compound microbial inoculant

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Amino Acid Fermentation Wastewater Analysis

[0026] It can be seen from the test reports of multiple batches of amino acid waste mother liquor (Table 1) that the waste mother liquor is highly acidic (pH 3.73) and contains a variety of amino acids, residual sugar, fermentation intermediates, ammonium salts and other components.

[0027] Table 1 Detection results of amino acid waste mother liquor

[0028]

[0029] Selection of the most suitable yeast single strain.

[0030] In order to investigate the degradation performance of a variety of different yeast strains on the reducing sugar in the waste amino acid mother liquor, the A1 (brewer's yeast ATCC9763), A2 (Rhodotorula viscosus ATCC32765), A3 (producer Four strains of Candida prion (ATCC22023) and AL (Angel yeast) were inoculated in the waste mother liquor of amino acids according to the inoculum amount of 10%, and the rotation speed of the shaker was controlled, and cultured for 48 hours. Samples were taken ever...

Embodiment 2

[0053] Comparing the effect of yeast compatibility on reducing reducing sugar.

[0054] Choose A with better hypoglycemic effect L 、A 2 、A 1 The three strains are compatible to improve the hypoglycemic efficiency.

[0055] Combination 1: A L +A 2；

[0056] Combination 2: A 2 +A 1；

[0057] Combination 3: A L +A 1；

[0058] Combination 4: A L +A 2 +A 1。

[0059] Optimization of growth conditions.

[0060] In order to determine the optimum growth pH value of the yeast strain and the optimum condition for hypoglycemia, the seed medium was prepared according to Table 7. After heating and dissolving, it was divided into test tubes, sterilized at 121°C for 20 minutes, and the prepared seed medium was taken out while it was hot; The strain was inoculated into the seed medium and cultivated to a concentration of 10 8 cfu / ml, each strain was mixed at a volume ratio of 1:1 to obtain a bacterial suspension. Then prepare the fermentation medium, add amino acid fermentati...

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Abstract

The invention belongs to the technical field of biology, and discloses a process for degrading organic matters in an amino acid fermentation waste liquid by utilizing a compound microbial inoculant. The compound microbial inoculant is characterized by comprising a combination of any two or more than two selected from the group consisting of beer yeast, rhodotorula glutinis and Angel yeast. The compound yeast is utilized to reduce the contents of reducing sugar and overall organic matters, so the concentration of burnt flavor generated in the guniting granulation process can be effectively reduced.

Description

technical field [0001] The invention belongs to the field of biological technology, and in particular relates to a process for degrading organic matter in amino acid fermentation waste liquid by using a compound bacterial agent. Background technique [0002] my country is a big country in the production and consumption of amino acids. A large amount of waste water containing amino acids is often discharged in the production of chemical, pharmaceutical, food and other industries. Taking the production of glutamic acid as an example, my country produces 2.3 million tons of glutamic acid every year, and discharges more than 19 million tons. Concentration acidic amino acid waste mother liquor. COD, SO in waste mother liquor 4 2- , NH 4 + -N and bacterial content are high, and it is acidic. After being discharged into the water body, it will significantly reduce the self-purification ability of the water body, hinder the growth of microorganisms in the water body, seriously po...

Claims

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Application Information

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IPC IPC(8): C02F3/34C12N1/18C12N1/16C12R1/865C12R1/645C02F101/38
CPCC02F3/347C12N1/18C12N1/16C02F2101/38
Inventor 王小平张传松程士清王伟涛
Owner HULUNBEIER NORTHEAST FUFENG BIOTECHNOLOGIES CO LTD