Gene for regulating and controlling synthesis of erucic acid using Tropaeolum majus and application thereof

A technique for synthesizing genes and nasturtiums, applied in the field of molecular biology

Active Publication Date: 2020-03-13
HEZE UNIV
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Problems solved by technology

[0004] At present, no other KCS gene members that can regulate erucic acid synthesis have been found. Development of new KCS gene members that regulate erucic acid synthesis. Research on KCS gene members of erucic acid synthesis and the genetic regulation mechanism of erucic acid in plants and genetic breeding of erucic acid source plants. of great significance

Method used

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  • Gene for regulating and controlling synthesis of erucic acid using Tropaeolum majus and application thereof
  • Gene for regulating and controlling synthesis of erucic acid using Tropaeolum majus and application thereof
  • Gene for regulating and controlling synthesis of erucic acid using Tropaeolum majus and application thereof

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Embodiment Construction

[0047] The present invention will be described in detail below in conjunction with embodiments and specific embodiments.

[0048] 1. Nasturtium nasturtium regulates erucic acid synthesis genes and their nomenclature

[0049] The materials of nasturtium seeds at different developmental stages were collected, RNAs were extracted by Trizol method, and the RNAs of materials at different developmental stages were mixed in equal quality, and the genes expressed during the development of nasturtium seeds were obtained by transcriptome sequencing and NCBI database blast homologous comparison. KCS gene, and its coding region sequence and encoded amino acid sequence were predicted.

[0050] The transcript sequence of the obtained KCS gene is shown in SEQ ID NO.1; its coding region sequence is shown in SEQ ID NO.2; and the encoded amino acid sequence is shown in SEQ ID NO.3.

[0051] The evolutionary relationship tree was constructed by constructing the evolutionary relationship tree wi...

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Abstract

The invention discloses a gene for regulating and controlling synthesis of erucic acid using Tropaeolum majus and application thereof, and belongs to the field of molecular biology. According to the gene for regulating and controlling the synthesis of erucic acid using Tropaeolum majus, the sequence of a coding region thereof is as shown in SEQ ID NO. 2, and the gene is named TM-KCS2 gene. The transcript sequence of the gene is as shown in SEQ ID NO. 1. The encoded amino acid sequence is as shown in SEQ ID NO. 3. The gene for regulating and controlling the synthesis of erucic acid using Tropaeolum majus is a new gene that promotes the synthesis of erucic acid. The gene can promote the synthesis of erucic acid. The invention provides a new gene resource for genetic breeding of erucic acid source plants and research on genetic regulation mechanism of plant erucic acid, and the gene is applied in regulating and controlling the synthesis of erucic acid.

Description

technical field [0001] The invention relates to the field of molecular biology, in particular to a nasturtium regulating erucic acid synthesis gene and its application. Background technique [0002] Erucic acid is a very long chain monounsaturated fatty acid. Due to its long molecular carbon chain, strong water resistance, and excellent lubricating properties, it is widely used in paint, plastics, cosmetics, medicine and other industries, and is known as an important fine chemical raw material in the 21st century. The synthesis of erucic acid in plants is catalyzed by the long-chain fatty acid carbon chain elongation enzyme complex anchored on the cell membrane, which is composed of four enzymes with different functions, among which β-ketoacyl-CoA synthase (β-Ketoacyl -CoA Synthase, KCS) is the rate-limiting enzyme that catalyzes the carbon chain elongation reaction of ultra-long-chain fatty acids, and controls the types of synthetic ultra-long-chain fatty acid products, an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/54C12N15/82A01H5/00A01H6/20
CPCC12N9/1029C12N15/8243C12Y203/01199
Inventor 周广灿王宜磊薛佳宇李稳陈晓吴雨含
Owner HEZE UNIV
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