Method for extracting dendrobium polysaccharides from Dendrobium denneanum
A technology for dendrobium polysaccharides and dendrobium polysaccharides is applied in the field of extracting dendrobium polysaccharides, and can solve the problems of large solvent consumption, complicated operation procedures, low extraction rate and the like
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Embodiment 1
[0020] A method for extracting dendrobium polysaccharides from dendrobium sheathes, comprising the following steps:
[0021] a. Fresh strip treatment: remove impurities from the freshly harvested Dendrobium sheath, wash and dry, cut into 4cm sections, and soak in softener for 1.5h;
[0022] b. Extraction: Grind the Dendrobium sheathes in step a. together with the softener into a dendrobium slurry, add twice the weight of acetone solution for extraction for 7 hours, and reclaim the organic solvent by distillation under reduced pressure, then add 6% antioxidant to obtain the extraction liquid;
[0023] c. Post-treatment: add miscellaneous cells and adsorbents to the extract, and stir at 110rpm for 1 hour to obtain a treatment solution;
[0024] d. Refining: filter the treatment solution to take the filtrate, evaporate and concentrate to make an extract, then further vacuum dry, pulverize and grind to obtain the dendrobium polysaccharide;
[0025] Further, the softening agent c...
Embodiment 2
[0032] A method for extracting dendrobium polysaccharides from dendrobium sheathes, comprising the following steps:
[0033] a. Fresh bar treatment: remove impurities from the freshly harvested Dendrobium sheath, wash and dry, cut into 5cm segments, and soak in softener for 2 hours;
[0034] b. Extraction: Grind the Dendrobium sheathes in step a. together with the softening agent into a dendrobium slurry, add twice the weight of acetone solution for extraction for 8 hours, and reclaim the organic solvent by distillation under reduced pressure, then add 8% antioxidant to obtain the extraction liquid;
[0035] c. Post-treatment: add miscellaneous cells and adsorbents to the extract, and stir at 120rpm for 1.5h to obtain a treatment solution;
[0036] d. Refining: filter the treatment solution to take the filtrate, evaporate and concentrate to make an extract, then further vacuum dry, pulverize and grind to obtain the dendrobium polysaccharide;
[0037] Further, the softening a...
Embodiment 3
[0044] A method for extracting dendrobium polysaccharides from dendrobium sheathes, comprising the following steps:
[0045] a. Fresh strip treatment: remove impurities from the freshly harvested Dendrobium sheath, wash and dry, cut into 3cm sections, and soak in softener for 1h;
[0046] b. Extraction: Grind the Dendrobium sheathes in step a. together with the softening agent into a dendrobium slurry, add twice the weight of acetone solution for leaching for 6 hours, and reclaim the organic solvent by distillation under reduced pressure, then add 5% antioxidant to obtain the extraction liquid;
[0047] c. Post-treatment: add miscellaneous cells and adsorbents to the extract, and stir at a rate of 90 rpm for 1 hour to obtain a treatment solution;
[0048] d. Refining: filter the treatment solution to take the filtrate, evaporate and concentrate to make an extract, then further vacuum dry, pulverize and grind to obtain the dendrobium polysaccharide;
[0049] Further, the soft...
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