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Multiplex fluorescence detection kit for 13-type high-risk HPV

A detection kit and multiple fluorescence technology, which is applied in the direction of recombinant DNA technology, microbial measurement/inspection, biochemical equipment and methods, etc., can solve the problems of low detection sensitivity and long detection time, and achieve the simple and easy operation of the detection method Effect

Active Publication Date: 2020-03-24
申友基因组研究院(南京)有限公司
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Problems solved by technology

[0004]The disadvantage of the existing high-risk human papillomavirus genotyping detection technology using fluorescent PCR is that the detection of a single type takes a long time, and the detection of multiple types Detection sensitivity is reduced due to interference between primers

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  • Multiplex fluorescence detection kit for 13-type high-risk HPV
  • Multiplex fluorescence detection kit for 13-type high-risk HPV
  • Multiplex fluorescence detection kit for 13-type high-risk HPV

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Embodiment Construction

[0077] The present invention will be further explained below in combination with specific embodiments.

[0078] Type 13 high-risk HPV multiplex fluorescent detection kit, including hot start Taq enzyme system, dUTP-UDG anti-pollution system, tailed primers, universal primers, fluorescent probes, negative control and positive control, the sequences of the tailed primers are as follows:

[0079] Primer 1 CAACGGGTCACGTAGCGAGATGAAATAGATGGTCCAGCT (SEQ ID NO: 1)

[0080] Primer 2 CAACGGGTCACGTAGCGACGCACAACCGAAGCGTAG (SEQ ID NO:2)

[0081] Primer 3 CAACGGGTCACGTAGCGAGAAGAAAACGATGAAATAGATGGA (SEQ ID NO: 3)

[0082] Primer 4 CAACGGGTCACGTAGCGACATTGTGTGACGTTGTGGTTC (SEQ ID NO: 4)

[0083] Primer 5 CAACGGGTCACGTAGCGAAGACCGTTGTGTCCAGAAGA (SEQ ID NO:5)

[0084] Primer 6 CAACGGGTCACGTAGCGAGGGTTTCAGTACGAGGTCTTCTC (SEQ ID NO: 6)

[0085] Primer 7 CAACGGGTCACGTAGCGATTTCGGGTCGTTGGGCA (SEQ ID NO: 7)

[0086] Primer 8 CAACGGGTCACGTAGCGAGGCGTTTTTACACGTCACAGT (SEQ ID NO: 8)

[0087] Primer 9 ...

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Abstract

The invention discloses a multiple fluorescence detection kit for 13-type high-risk HPV, which comprises a hot start Taq enzyme system, a dUTP-UDG anti-pollution system, a tailing primer, a universalprimer, a fluorescence probe, a negative control and a positive control, and the fluorescence probe is an HPV specific probe; the kit disclosed by the invention is suitable for qualitatively detecting13 high-risk HPV genotypes capable of causing cervical cancer in cervical exfoliated cells and genitourinary tract secretion samples, and typing 16 and 18 genotypes; according to the kit, a homologous tailing system is applied, so that the interference among a plurality of primers is reduced, 13 HPV genotypes are detected in the same fluorescent PCR amplification system, the detection flux of thedetection kit is high, and the screening cost is greatly reduced.

Description

technical field [0001] The invention relates to a multiple fluorescence detection kit for type 13 high-risk HPV, in particular to a multiple high-risk HPV fluorescence detection kit based on a HAND system, which can be used to type 16 and 18 genotypes. Background technique [0002] Cervical cancer is the most common gynecological malignancy. The high-incidence age of carcinoma in situ is 30-35 years old, and the high-incidence age of invasive carcinoma is 45-55 years old. Currently, the onset tends to be younger. Early screening, detection and treatment of cervical cancer is the key to effective prevention and treatment of cervical cancer. [0003] Studies have shown that the main pathogen of cervical cancer is human papillomavirus (Human Papillomavirus, HPV), which belongs to Papovaviridae, Papillomavirus A genus, spherical DNA virus, which can cause the proliferation of human skin and mucous membrane squamous epithelium. At present, there are more than 190 types of HPV i...

Claims

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Application Information

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IPC IPC(8): C12Q1/70C12Q1/6851C12N15/11C12R1/93
CPCC12Q1/708C12Q1/6851C12Q2600/16C12Q2600/166C12Q2563/107C12Q2537/143C12Q2545/101
Inventor 董辉金维荣秦红友顾立江
Owner 申友基因组研究院(南京)有限公司
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