Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for extracting abalone hepatopancreas metabolites

A technology of hepatopancreas and metabolites, applied in the field of molecular biology, can solve problems such as being unsuitable for high-throughput, large-scale use, ineffective removal of polysaccharides and pigments, affecting the quantification of important metabolites, etc., to eliminate polysaccharides and pigments. The effect of interference, high recovery rate and low cost

Active Publication Date: 2020-03-27
SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
View PDF5 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method cannot effectively remove polysaccharides and pigments in abalone hepatopancreas
Polysaccharides and pigments will cover up the signals of some important metabolites in the NMR spectrum, which will seriously affect the quantification of these important metabolites, making subsequent analysis impossible
Although polysaccharides and pigments can be effectively removed using ultrafiltration centrifuge tubes, ultrafiltration centrifuge tubes are expensive and not suitable for high-throughput, large-scale use

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for extracting abalone hepatopancreas metabolites
  • Method for extracting abalone hepatopancreas metabolites

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 Using the method of the present invention to extract abalone hepatopancreas metabolites

[0034] The specific method includes the following steps:

[0035] S1. Weigh 300 mg abalone hepatopancreas and place in a 5 mL centrifuge tube;

[0036] S2. Add 1.2 mL of methanol and 0.6 mL of water to the centrifuge tube in step S1, and add steel balls, and then place it in a low-temperature tissue grinder and vibrate at 4 °C to break;

[0037] S3. Add 1.2 mL of chloroform and 1.2 mL of water to the centrifuge tube in step S2, vortex at a speed of 2500 rpm for 1 min, and place it on ice for 10 min;

[0038] S4. Centrifuge at 10000 g for 10 min at 4 °C after standing still, and transfer the upper layer solution to another centrifuge tube;

[0039] S5. Put the upper layer solution obtained in step S4 into a freeze-dried powder by vacuum freeze-drying in a vacuum freeze dryer at -80°C after quick-freezing with liquid nitrogen;

[0040] S6. Add 0.9 mL methanol and 0.1 mL ...

Embodiment 2

[0047] Example 2 Using the method of the present invention to extract hepatopancreatic metabolites from abalone

[0048] The specific method includes the following steps:

[0049] S1. Weigh 300 mg abalone hepatopancreas and place in a 5 mL centrifuge tube;

[0050] S2. Add 1.2 mL of methanol and 0.6 mL of water to the centrifuge tube in step S1, and add steel balls, and then place it in a low-temperature tissue grinder and vibrate at 4 °C to break;

[0051] S3. Add 1.2 mL of chloroform and 1.2 mL of water to the centrifuge tube in step S2, vortex at a speed of 2500 rpm for 1 min, and place it on ice for 10 min;

[0052] S4. Centrifuge at 10000 g for 10 min at 4 °C after standing still, and transfer the upper layer solution to another centrifuge tube;

[0053] S5. Put the upper layer solution obtained in step S4 into a freeze-dried powder by vacuum freeze-drying in a vacuum freeze dryer at -80°C after quick-freezing with liquid nitrogen;

[0054] S6. Add 0.9 mL methanol and ...

Embodiment 3

[0061] Example 3 Using the method of the present invention to extract abalone hepatopancreas metabolites

[0062] The specific method includes the following steps:

[0063] S1. Weigh 300 mg abalone hepatopancreas and place in a 5 mL centrifuge tube;

[0064] S2. Add 1.2 mL of methanol and 0.6 mL of water to the centrifuge tube in step S1, and add steel balls, and then place it in a low-temperature tissue grinder and vibrate at 4 °C to break;

[0065] S3. Add 1.2 mL of chloroform and 1.2 mL of water to the centrifuge tube in step S2, vortex at a speed of 2500 rpm for 1 min, and place it on ice for 10 min;

[0066] S4. Centrifuge at 10000 g for 10 min at 4 °C after standing still, and transfer the upper layer solution to another centrifuge tube;

[0067] S5. Put the upper layer solution obtained in step S4 into a freeze-dried powder by vacuum freeze-drying in a vacuum freeze dryer at -80°C after quick-freezing with liquid nitrogen;

[0068] S6. Add 0.9 mL methanol and 0.23 mL...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the technical field of molecular biology, in particular to a method for extracting hepatopancreas metabolites of abalones, which comprises the following steps: weighing a hepatopancreas sample, adding a methanol aqueous solution, oscillating and crushing, adding chloroform and water, whirling, standing at low temperature, centrifuging, and collecting a first supernatant; freeze-drying the first supernatant, adding a methanol aqueous solution, carrying out ultrasonic treatment, standing, centrifuging, and collecting a second supernatant; collecting the residual precipitate in the second supernatant, adding a methanol aqueous solution, carrying out ultrasonic treatment, standing, centrifuging, and collecting a third supernatant; mixing and freeze-drying the second supernatant and the third supernatant; addition of phosphate buffer, according to the abalone hepatopancreas metabolite extraction method, interference of polysaccharide and pigment in abalone hepatopancreas is thoroughly eliminated, operation is easy, cost is low, stability is high, the recovery rate is high, and the abalone hepatopancreas metabolite extraction method has a reference effect on extraction of other animal and plant tissue metabolite with high polysaccharide and pigment content.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to a method for extracting abalone hepatopancreas metabolites. Background technique [0002] Abalone is known as the "crown of the eight treasures" of marine products. It has high nutritional and medicinal value. It is an important economically cultured shellfish in the coastal areas of my country, and it is also an ideal marine environment monitoring indicator organism. The hepatopancreas is an important digestive tissue of abalone, and it is also an important immune tissue against external environmental stress. The extraction of metabolites in the hepatopancreas is an important link in the study of metabolomics. In nuclear magnetic resonance (NMR)-based metabolomics studies, liquid-liquid extraction using methanol / chloroform / water as the extraction solvent is most widely used in the extraction of polar metabolites. However, this method cannot effectively remove polysac...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N24/08
CPCG01N1/28G01N24/08
Inventor 卢洁姚托叶灵通王江勇
Owner SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com