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A kind of bacillus subtilis, microbial agent and application thereof

A technology of Bacillus subtilis and microbial agent, applied in the field of microorganisms, can solve the problems of insufficient and effective removal of organic pollutants, and achieve the effect of low treatment cost

Active Publication Date: 2021-07-30
HAINAN JINYUFENG BIOLOGICAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to overcome the defect that the existing sewage treatment methods cannot meet the requirements of fully and effectively removing organic pollutants, the invention provides a Bacillus subtilis

Method used

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  • A kind of bacillus subtilis, microbial agent and application thereof
  • A kind of bacillus subtilis, microbial agent and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0038] The Bacillus subtilis with the preservation number CGMCC No. 16827 was inoculated from the preserved slant into the beef extract peptone culture solution, shaken at 37° C. and 180 rpm for 12 hours, and the seed solution was prepared. Add 100mL of the above seed solution into 100L of fermentation medium, cultivate at 30°C, take samples during the cultivation and observe by direct counting under a microscope until the number of viable Bacillus subtilis per gram of culture solution is 10 10 CFU, the resulting culture solution is the microbial agent of this embodiment.

Embodiment 2

[0040] Bacillus subtilis, Thiobacillus denitrificans ATCC25259, Pseudomonas fluorescens CCTCC NO: M209107 and Bacillus CCTCC M2014602 with the preservation numbers of CGMCC No. 16827 were respectively made into seed liquid according to the method in Example 1. Add 100mL of the above-mentioned various seed liquids to 100L fermentation medium respectively, cultivate at 30°C, take samples during the cultivation process and observe by direct counting under a microscope until the total number of viable bacteria per gram of culture liquid is 10 10 CFU, the obtained culture solution is mixed according to the CFU ratio of 1:1:1:1, which is the microbial agent of this embodiment.

Embodiment 3

[0042] Bacillus subtilis, Thiobacillus denitrificans ATCC25259, Pseudomonas fluorescens CCTCC NO: M209107 and Bacillus CCTCC M2014602 with the preservation numbers of CGMCC No. 16827 were respectively made into seed liquid according to the method in Example 1. Add 100mL of the above-mentioned various seed liquids to 100L fermentation medium respectively, cultivate at 30°C, take samples during the cultivation process and observe by direct counting under a microscope until the total number of viable bacteria per gram of culture liquid is 10 10 CFU, the obtained culture solution is mixed according to the CFU ratio of 1:0.4:0.6:0.2, which is the microbial agent of this embodiment.

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Abstract

The present disclosure relates to a Bacillus subtilis and application thereof. The deposit number of Bacillus subtilis of the present disclosure is CGMCC No.16827. The present disclosure also provides a microbial agent, which contains the above-mentioned Bacillus subtilis as an active ingredient. The present disclosure also provides the application of the above-mentioned Bacillus subtilis and the above-mentioned microbial agent in treating urban sewage. Through the above technical solution, the present disclosure can more quickly and effectively remove and treat organic matter in urban sewage.

Description

technical field [0001] The disclosure belongs to the technical field of microorganisms, and in particular relates to a bacillus subtilis, a microbial agent and applications thereof. Background technique [0002] Urban sewage refers to domestic sewage, industrial wastewater and runoff sewage within urban areas. In addition to containing a large amount of organic matter, germs and viruses in urban sewage, due to the high development of industry, the water volume of industrial wastewater (accounting for about 60-80% of the total urban sewage), the water quality is becoming more and more complex and the pollution of runoff sewage is becoming more and more serious, making urban sewage Contains various types and degrees of various toxic and harmful pollutants. Urban sewage generally contains organic pollutants (indicated by COD and BOD5), including carbohydrates, proteins, amino acids, fatty acids, oils, esters and other substances. [0003] Existing sewage treatment methods can...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/125
CPCC02F3/34C12N1/20C12N1/205C12R2001/125
Inventor 吴蓓蕾蒋细良李梅
Owner HAINAN JINYUFENG BIOLOGICAL ENG
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