LN-229 cell line with low expression of human phosphatase and tensin homolog (PTEN) protein and construction method for LN-229 cell line

A low-expression technology of LN-229, applied in the field of biomedicine, can solve the problems of cell damage, lack of stable, high-efficiency and low-expression PTEN cell line models, and low transfection efficiency
CN110938601AInactive Publication Date: 2020-03-31DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
Publication Date
2020-03-31
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a construction method for a LN-229 cell line with low expression of phosphatase and tensin homolog (PTEN). The stable LN-229 cell line with low expression of PTEN is constructed by utilizing a lentivirus system; shPTEN and lentivirus packaging plasmids are transfected into HEK293T cells; a virus supernatant is collected to infect LN-229 cells; the transfected LN-229 cellsare screened with puromycin to obtain the stable LN-229 cell line with low expression of PTEN; and finally, tested by a Western blot technique, the expression of PTEN of cell strains is decreased by more than 80%, and thus, the cell line with low expression of PTEN is successfully constructed. The cell line can serve as an ideal cell line for researching the molecular action mechanism and the participating metabolic regulation effect of PTEN in glioma.
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Description

technical field

[0001] The invention relates to the construction of an LN-229 cell line stably and lowly expressing PTEN protein and a construction method thereof, and relates to the field of biomedicine. Background technique

[0002] PTEN catalyzes the dephosphorylation of PtdIns(3,4,5)P3. PTEN loss occurs in a variety of tumors, and PTEN loss exists in about 75% of gliomas. Glioma patients with PTEN deletion generally have a shorter survival period. Therefore, the study of PTEN is of great significance to the study of new tumor markers and marker proteins of cell metabolism disorders in glioma, and it can also be used for adjuvant treatment such as early clinical diagnosis and prognosis judgment of glioma.

[0003] Studying the molecular mechanism of PTEN requires the construction of stable and low-expression cell lines of PTEN as cell models, but there is currently a lack of cell line models with stable, high-efficiency and low-expression of PTEN in glioma. The constru...

Claims

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