Method for determining earthy-smell substances in fish flesh

Abstract

The invention discloses a method for determining earthy-smell substances in fish flesh, which comprises the following steps: taking fish flesh prepared by taking a cis-decahydro-1-naphthol solution asan internal standard as a sample, extracting geosmin and 2-methylisoborneol in the sample by adopting a headspace solid-phase microextraction method, and performing a determination process by adopting a gas chromatography-mass spectrometry method. A headspace solid-phase microextraction method is adopted to extract earthy-smell substances in the fish flesh, cis-decahydro-1-naphthol is taken as aninternal standard, and qualitative and quantitative determination is carried out by a gas chromatography-mass spectrometry method, so that the detection method of the geosmin and the 2-methylisoborneol in the fish flesh is established, and a detection method basis is provided for removing peculiar-smell substances in cultured aquatic products.

Description

technical field [0001] The invention relates to a method for measuring earthy smell substances in fish meat, in particular to extraction, enrichment and detection methods of earthy smell substances geosmin and 2-methylisoborneol in fish meat, and belongs to the field of food detection. Background technique [0002] Aquatic products are one of the main sources of high-quality animal protein in human food. my country is the largest country in aquaculture. In 2018, the total output of aquatic products in the country was 64.5766 million tons, of which the aquaculture output was 49.9106 million tons. However, the peculiar smell of some aquaculture aquatic products is difficult for consumers to accept. It is a problem that has plagued the aquaculture industry for a long time and directly affects the consumption of aquatic products and the economic benefits of aquaculture. According to research reports, geosmin (geosmin) and 2-methylisoborneol are the two most common compounds tha...

AI Technical Summary

Problems solved by technology

[0004] Due to the complex composition of fish meat, there are many types of volatile flavor substances. As a kind of volatile components that affect the quality of fish meat, earthy smell substances have the characteristics of difficult extraction and adsorption, many interfering substances, and complex sample matrices, making detection difficult.

In the prior art, for the extraction and detection method of geosmin and 2-methylisoborneol in fish tissue, as document [14-16] The microwave distillation-solid-phase microextraction-gas chromatography-mass spectrometry method was reported for the determination of geosmin and 2-methylisoborneol in the muscle of catfish and tilapia. Difficult to standardize, poor repeatability and stability

Another example is E.D. Ruan [17] SBSE-TD-GC-MS (stir bar absorption extraction-thermal desorption-gas chromatography-mass spectrometry) was used to determine the content of geosmin and 2-methylisoborneol in Atlantic salmon muscle. The thermal desorption device requires a large investment in equipment, and the recovery rate of geosmin is 93-96%, but the recovery rate of 2-methylisoborneol is only 23-30%, and the recovery rate of 2-methylisoborneol is The rate is too low to meet the detection requirements

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