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Method for enriching jujube witches' broom phytoplasma by using catharanthus roseus

A technology for enriching jujube madness and periwinkle, applied in microorganism-based methods, biochemical equipment and methods, flower cultivation, etc., can solve problems such as unseen

Inactive Publication Date: 2020-04-03
HENAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no relevant report on how to use periwinkle to enrich the phytoplasma of jujube madness

Method used

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  • Method for enriching jujube witches' broom phytoplasma by using catharanthus roseus
  • Method for enriching jujube witches' broom phytoplasma by using catharanthus roseus
  • Method for enriching jujube witches' broom phytoplasma by using catharanthus roseus

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Experimental program
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Effect test

Embodiment 1

[0039] A kind of method utilizing periwinkle to enrich jujube mad disease phytoplasma, concrete steps are as follows:

[0040] (1) Cultivate periwinkle seedlings as rootstock for grafting

[0041] After the periwinkle seeds are sown, they are normally cultivated, and the periwinkle seedlings grown for 30-40 days after sowing are used as grafting materials, and the ground diameter of 5-8mm is preferably used as grafting materials;

[0042] (2) Grafting transfection

[0043]From the jujube tree that is sick with jujube mad disease, choose the jujube mad disease branch, use the jujube mad disease branch as the scion, and adopt the abdominal grafting method (such as figure 2 Shown) Jujube mad disease branch is grafted on the stock periwinkle seedling. Before the specific grafting operation, such as figure 1 As shown, prepare the rootstock, jujube mad branch scion, and some operating tools: grafting tape, 75% alcohol and grafting knife, wherein, the specific operation steps of ...

Embodiment 2

[0051] A kind of method utilizing periwinkle to enrich jujube mad disease phytoplasma, concrete steps are as follows:

[0052] (1) Cultivate periwinkle seedlings as rootstock for grafting

[0053] After the periwinkle seeds are sown, they are normally cultivated, and the periwinkle seedlings grown for 30-40 days after sowing are used as grafting materials, and the ground diameter of 5-8mm is preferably used as grafting materials;

[0054] (2) Grafting transfection

[0055] From the jujube tree that is sick with jujube mad disease, choose the jujube mad disease branch, use the jujube mad disease branch as the scion, and adopt the splitting method (such as image 3 Shown) Jujube mad disease branch is grafted on the stock periwinkle seedling. Before the specific grafting operation, with embodiment 1, wherein, the specific operation steps of split grafting method are as follows:

[0056] A. Disinfect the grafting knife, use the grafting knife to remove the top of the periwinkle...

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Abstract

The invention discloses a method for enriching jujube witches' broom phytoplasma by using catharanthus roseus. The method uses catharanthus roseus as a rootstock, and a jujube witches' broom branch asa scion for grafting, then a grafted plant is moved into an artificial climate control room for cultivation, first dark culture is performed for 1 d under conditions of 23 DEG C and 85% relative humidity, and then culture is performed until the onset of illness under conditions of 16 h photoperiod, 23 DEG C and 65% relative humidity. The method relatively well achieves the enrichment of jujube witches' broom phytoplasma, so that problems such as low content of phytoplasma in an original host, separation difficulty and cumbersome operation in the existing jujube witches' broom phytoplasma separation research are relatively well solved, and thereby the foundation for the in-depth research of jujube witches' broom phytoplasma, and how to prevent and treat jujube witches' broom is laid.

Description

technical field [0001] The invention belongs to the technical field of phytoplasma preservation, and in particular relates to a method for preserving and enriching the phytoplasma of jujuba disease by utilizing periwinkle flowers. Background technique [0002] Jujube witches' broom, caused by phytoplasma, is an epidemic disease widely spread in East Asia. Because it is difficult to prevent and control, it has the characteristics of fast transmission, high pathogenicity, and destructiveness, so it is also called "jujube cancer". Once jujube trees are infected with jujube madness, symptoms such as arbuscular branches, dwarfing, and sterility will appear, which will cause serious economic losses to the jujube industry. Studies have shown that the pathogen of jujube madness, phytoplasma, obligately parasitizes in the phloem and young tissues of plants, and cannot be cultured in vitro, which brings great difficulties to the research of this disease. However, since the pathogen ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G22/60A01G2/30C12N1/20C12R1/01
CPCA01G2/30A01G22/60C12N1/20
Inventor 李继东陈鹏冯建灿叶霞郑先波谭彬程钧王伟
Owner HENAN AGRICULTURAL UNIVERSITY
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