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Method for regulating and controlling synthesis stability of mixed flora polyhydroxyalkanoate by utilizing azithromycin

A technology of polyhydroxyalkanoate and azithromycin, which is applied in the direction of adding compounds to stimulate growth and fermentation, can solve the problems of poor PHA process stability and the degree of influence of the enrichment section of PHA synthetic bacteria, and achieve rapid inhibition of sludge loss, The effect of preventing biomass and PHA yield reduction and reducing losses

Active Publication Date: 2020-04-03
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The purpose of the present invention is to solve the problem that the process stability of PHA produced by mixed flora is poor, and the enrichment section of PHA synthetic bacteria is most easily affected and to the greatest extent. The stability method, which is based on the traditional PHA synthetic bacteria enrichment reactor, can quickly inhibit the sludge loss caused by sludge bulking within 1-2 SRTs, and improve the sludge settling performance. In addition, this method can not Change other operating parameters within a reasonable range to reduce process adjustment costs while still maintaining PHA production capacity during the recovery phase

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  • Method for regulating and controlling synthesis stability of mixed flora polyhydroxyalkanoate by utilizing azithromycin
  • Method for regulating and controlling synthesis stability of mixed flora polyhydroxyalkanoate by utilizing azithromycin

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specific Embodiment approach 1

[0015] Specific embodiment 1: This embodiment records a method for using azithromycin to regulate the synthesis stability of mixed flora polyhydroxyalkanoate. The method is as follows: figure 1 shown, including the following steps:

[0016] 1. Add azithromycin to the enrichment reactor for the synthesis of PHA by the mixed flora that is lost due to sludge bulking. The enrichment reactor is at an organic load of 0.8-4kg COD / m 3 / d operation, and the rest of the operating parameters are within the appropriate parameter range of the traditional PHA mixed flora enrichment reactor;

[0017] 2. Detection of enrichment reactor sludge sedimentation ratio SV 30 , calculate the sludge volume index SVI and carry out the sludge microscope inspection (the purpose of the microscope inspection is to observe the sludge form);

[0018] 3. If any of the following two situations occurs: (1) SV 30 If it is higher than 60%, (2) SVI is greater than 150, then repeat the above steps 1 and 2, and j...

specific Embodiment approach 2

[0020] Specific embodiment two: a method for using azithromycin to regulate the synthesis stability of mixed flora polyhydroxyalkanoate described in specific embodiment one, in step one, the dosage of said azithromycin is: final concentration 1~10mg / L.

[0021] Specific embodiment three: a method for using azithromycin to regulate the synthesis stability of polyhydroxyalkanoate of mixed flora described in specific embodiment two, in step one, the dosage of said azithromycin is: the final concentration is 5 mg / L.

[0022] Embodiment 4: A method for using azithromycin to regulate the synthesis stability of polyhydroxyalkanoate of mixed flora described in Embodiment 1. In step 1, the enrichment reactor is kept at 15-25°C for continuous Aeration or mechanical stirring.

[0023] Embodiment 5: In Embodiment 1, a method for using azithromycin to control the synthesis stability of polyhydroxyalkanoate of mixed flora, in step 1, the operation period of the enrichment reactor is 6-24 ...

specific Embodiment approach 9

[0027] Embodiment 9: In Embodiment 1, a method for using azithromycin to regulate the synthesis stability of polyhydroxyalkanoate of mixed flora, in step 3, the sludge residence time is 1.5 SRT.

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Abstract

The invention discloses a method for regulating and controlling synthesis stability of mixed flora polyhydroxyalkanoate by utilizing azithromycin, which belongs to the technical field of biodegradableplastic synthesis and waste resource recovery, and comprises the following steps: adding azithromycin into a PHA synthetic bacterium enrichment reactor with sludge bulking, detecting the sludge settling ratio SV30 of the reactor, and calculating the sludge volume index SVI; if the SV30 is lower than 60%, the SVI is lower than 150, and the duration exceeds one SRT, recovering the normal operationof the azithromycin; and if the expansion is not obviously improved, continuously adding the azithromycin until all indexes meet the requirements, and the normal operation is recovered. According to the method, the instability of a domestication reactor and even serious sludge loss caused by expansion of an extracellular polymeric substance (EPS) can be quickly recovered in a short time, so that the reduction of biomass and PHA yield caused by process instability is prevented in time, and the operation loss is reduced.

Description

technical field [0001] The invention belongs to the technical field of biodegradable plastic synthesis and waste resource recovery, and specifically relates to a method for regulating the synthesis stability of polyhydroxyalkanoate of mixed flora by using azithromycin. Background technique [0002] Traditional industrialization and modernization have given birth to polymer materials represented by petrochemical plastics. Although their large-scale application has brought great convenience to human society, it has also brought serious damage to the natural environment. Traditional plastic products that are difficult to biodegrade that have been produced and used by humans for many years are seriously threatening the marine ecological environment and soil ecological environment. In recent years, marine plastic pollution and microplastic pollution, which have attracted increasing attention, show that the pollution caused by traditional petrochemical plastics will not only harm ...

Claims

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Application Information

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IPC IPC(8): C12P7/62C12N1/38
CPCC12P7/625C12N1/38
Inventor 温沁雪刘少蛟陈志强
Owner HARBIN INST OF TECH