A high-speed countercurrent chromatographic separation column

Abstract

The invention discloses a high-speed counter-current chromatographic separation column, which relates to the technical field of counter-current chromatography, and comprises a planetary shaft, a bobbin and a pressure ring; The diameter of the lower cylinder is smaller than the diameter of the upper cylinder, and one end of the lower cylinder and the planetary shaft is connected by a thread; the screw thread direction matches the rotation direction of the planetary shaft so that when the planetary shaft rotates, the planetary shaft and the bobbin The connection is tighter; the crimping ring is cylindrical, and the crimping ring and the upper barrel are connected by threads, and the thread direction matches the rotation direction of the planetary shaft so that the crimping ring and the bobbin are connected when the planetary shaft rotates more closely. The high-speed countercurrent chromatographic separation column can be applied to higher rotation speeds, and is tightly connected and easy to disassemble under high-speed operation; at the same time, the winding volume is effectively increased without changing the size of the separation column; and the operation is safer and more reliable. Better fluidity, better separation effect and higher separation efficiency.

Description

technical field [0001] The invention relates to the technical field of countercurrent chromatography, in particular to a high-speed countercurrent chromatography separation column. Background technique [0002] Countercurrent chromatography technology is a continuous and efficient liquid-liquid partition chromatography separation technology. It generates a two-dimensional force field through synchronous planetary motion of simultaneous revolution and rotation, and retains one of the two phases as the stationary phase. During high-speed rotation Realize separation and extraction. [0003] The structure of the countercurrent chromatographic separation column in the prior art is as follows: figure 1 , figure 2 As shown, it includes an integrated bobbin 1 , a planetary shaft 4 and a tension member 5 . The upper end of the integrated bobbin 1 is provided with two quick connectors 6. During installation, the threading operation of the separation column is completed first. Spec...

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Problems solved by technology

[0004] The existing countercurrent chromatographic column has the following defects in use: First, the connection between the integrated bobbin 1 and the planetary shaft 4 through the wedging and tightening of the expansion member 5 is poor in reliability. , the position between the integrated bobbin 1 and the planetary shaft 4 will change relative to each other, resulting in a misalignment between the expansion and tightening hole 8 and the screw 7, making disassembly difficult or even impossible. Therefore, in actual use, usually It is necessary to reduce the working speed to below 2000r / min, and its separation ability and separation efficiency are relatively low; second, due to the connection by expansion and tightening, in order to ensure the structural strength, the winding space of the integrated bobbin 1 is small, and its unit The amount of separation time is limited. To improve the separation efficiency, it is often necessary to use a preparation column with a larger diameter, which will lead to an increase in the volume of the entire chromatographic separation equipment. To achieve the same speed, the strength of the entire transmission system will be higher. Third, in the above-mentioned threading operation, when the polytetrafluoroethylene tube penetrates into the lower end of the quick connector 6 to complete the connection, it is necessary to tighten the hollow screw at the lower end of the quick connector 6, because the polytetrafluoroethylene tube The screw needs to be penetrated first, and then tightened. At the same time, due to the limited operating space, the inner hexagonal countersunk screw cannot be used, and only the outer hexagonal screw can be used, which leads to more protrusions on the head of the screw. The longer part of the tube will be bent, and the pressure of the PTFE tube in the bent part will increase, while the inner diameter of the PTFE tube is only 0.8mm, and it is prone to leakage when it is under high pressure for a long time. This leads to the failure of the entire separation column; fourth, for the convenience of disassembly, a disassembly hole needs to be reserved on the top surface of the integrated bobbin, and there is no redundant position on the surface to set a dynamic balance hole, and the dynamic balance of the separation column cannot be adjusted. It is not conducive to the dynamic balance of the separation column, which limits the increase in the speed of the separation column. At the same time, the manufacturing precision of the separation column is extremely high, which makes its production difficult and high in production costs.

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