Carbon dot fluorescent probe for calcium ion detection, and preparation method thereof

A technology of carbon dot fluorescence and calcium ions, which is applied in the direction of fluorescence/phosphorescence, chemical instruments and methods, measuring devices, etc., can solve the problem of undisclosed reliable technical solutions for calcium ion detection, and achieve cheap raw materials, simple and safe preparation process Effect

Pending Publication Date: 2020-04-07
SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no reliable technical solution for applying carbon dots to calcium ion detection.

Method used

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  • Carbon dot fluorescent probe for calcium ion detection, and preparation method thereof
  • Carbon dot fluorescent probe for calcium ion detection, and preparation method thereof
  • Carbon dot fluorescent probe for calcium ion detection, and preparation method thereof

Examples

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Effect test

Embodiment 1

[0025] A carbon-dot fluorescent probe for calcium ion detection comprises carbon dots and ethylene glycol bis(2-aminoethyl ether)tetraacetic acid (EGTA) modified on the carbon dots. The carbon dot fluorescent probe prepared by modifying (EGTA) on the carbon dots in the present invention can be used for calcium ion detection.

Embodiment 2

[0027] A preparation method for a carbon dot fluorescent probe for calcium ion detection, comprising the following steps:

[0028] 1) Prepare carbon dots;

[0029] 2) Add ethylene glycol bis(2-aminoethyl ether) tetraacetic acid to the prepared carbon dots to obtain carbon dots modified with ethylene glycol bis(2-aminoethyl ether) tetraacetic acid, which is the Carbon dot fluorescent probe for calcium ion detection.

[0030] In a preferred embodiment, refer to figure 1 , the specific methods include:

[0031] 1) Preparation of carbon dots: Weigh 1.5g of citric acid, dissolve in 40mL of deionized water, stir well to dissolve completely, add 1mL of ethylenediamine with a pipette gun; after stirring well, transfer the solution into 80mL of polytetrafluoroethylene lining The reaction kettle with the bottom was reacted at 180°C for 8 hours to obtain carbon dots;

[0032] 2) Modification of EGTA: Add 1 g of ethylene glycol bis(2-aminoethyl ether) tetraacetic acid to the reactor, ...

Embodiment 3

[0034] Perform performance test on the carbon dots modified by EGTA:

[0035] refer to figure 2 , is a transmission electron microscope image of EGTA-modified carbon dots. It can be seen from the figure that the size of each carbon dot is about 4-5nm, and the particle size is uniform and the dispersion is good. From high-resolution transmission electron microscope photos ( figure 2 It can be seen that the carbon dots have an obvious lattice structure.

[0036] refer to image 3 , is the infrared spectrum of the carbon dot modified by EGTA. It can be seen from the figure that the carbon dot is at 3240cm -1 , 2935cm -1 , 1646cm -1 , 1530cm -1 and 1374cm -1 There is an absorption peak at 3240cm -1 Represents the stretching vibration of -OH, 2935cm -1 Represents the stretching vibration of C-H, 1646cm -1 Represents the vibration of -CO-NH, 1530cm -1 and 1374cm -1 They are the stretching vibrations of N-H and C=O, respectively.

[0037] refer to Figure 4 , is the ...

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Abstract

The invention discloses a carbon dot fluorescent probe for calcium ion detection, and a preparation method thereof. The carbon dot fluorescent probe comprises a carbon dot and ethylene glycol bis(2-aminoethyl ether)tetraacetic acid modified on the carbon dot. The preparation method comprises the following steps: 1) preparing the carbon dot; and 2) adding the ethylene glycol bis(2-aminoethyl ether)tetraacetic acid into the prepared carbon dot to obtain the carbon dot modified with ethylene glycol bis(2-aminoethyl ether)tetraacetic acid, namely the carbon dot fluorescent probe for calcium ion detection. The carbon dot fluorescent probe for calcium ion detection can detect the concentration of calcium ions in cells, and the carbon dot calcium ion probe prepared by the method has the advantages of cheap raw materials, simple and safe preparation process, and suitableness for large-scale production.

Description

technical field [0001] The invention relates to the technical field of analytical chemistry and nanometer materials, in particular to a carbon dot fluorescent probe for calcium ion detection and a preparation method thereof. Background technique [0002] Calcium ion is an important metal to ensure the normal operation of life. From the generation of life to the performance of various functions of cells, they are more or less regulated by calcium ions. The concentration change of calcium ions in cells is closely related to various physiological and pathological phenomena. Therefore, accurate measurement of intracellular calcium ion concentration and its transformation has become an important research topic in the fields of chemistry, biology and medicine. Numerous techniques for analyzing the concentration of calcium ions in cells and subcells have also emerged as the times require. Fluorescent probe method plays an important role in the detection of intracellular concentra...

Claims

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Application Information

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IPC IPC(8): C09K11/65C09K11/02G01N21/64
CPCC09K11/65C09K11/025G01N21/643
Inventor 李力董文飞梅茜葛明锋常智敏邵昊华尤倩楠岳娟曹磊
Owner SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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