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Label quality control method and device

A labeling and quality control technology, applied in the field of high-throughput sequencing, can solve the problems of difficult and accurate quality control of labels, and achieve the effect of low detection cost and simplified workload

Active Publication Date: 2020-04-07
BEIJING USCI MEDICAL LAB CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0015] The main purpose of the present invention is to provide a method and device for label quality control to solve the problem that it is difficult to achieve accurate quality control for labels in the prior art

Method used

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  • Label quality control method and device
  • Label quality control method and device

Examples

Experimental program
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Effect test

Embodiment 1

[0048] As mentioned in the background art, the existing method is difficult to perform quality control on the contamination of the index used in the mixed sample sequencing of the library. In order to improve this situation, the applicant of the present application improved the existing method. In a preferred embodiment, a method for label quality control is provided, such as image 3 As shown, the method includes:

[0049] Step S101, construct M libraries for M known sequences, each library contains one or more tags, each known sequence and each tag form a known tag-sequence combination, and there are N types of M libraries Tags, any two or more libraries contain at most one same tag, where M and N are natural numbers, M≤N;

[0050] Step S102, putting the M libraries in the same lane for sequencing to obtain the original sequencing data;

[0051] Step S103, by analyzing whether there is a tag-sequence combination inconsistent with known tag-sequence combinations in the raw ...

Embodiment 2

[0094] This embodiment also provides a device for label quality control, such as Figure 4 As shown, the device includes: a preset combination module 20, a sequencing module 40 and a pollution judgment module 60, wherein the preset combination module 20 is used to construct M libraries for M known sequences, and each library contains a or multiple tags, each known sequence and each tag form a known tag-sequence combination, there are N tags in M ​​libraries, and any two or more libraries contain at most 1 identical tag, where , M and N are natural numbers, M≤N; the sequencing module 40 is used to place M libraries in the same lane for sequencing to obtain the original sequencing data; the pollution judgment module 60 is used to analyze whether there is a The known tag-sequence combinations do not match the tag-sequence combinations to determine whether there is contamination in the N tags.

[0095] The device for quality control of the above-mentioned tags performs quality in...

Embodiment 3

[0105] Oligo sequence: artificially synthesized nucleic acid sequence. The specific design method is: randomly generate sequences, each 150bp, and then compare it with the human reference genome sequence. The sequence that cannot be compared is used as the Oligo sequence here. The sequences selected in this example are 23 non-repetitive sequences selected from the sequences that meet the above design conditions (see the sequence listing for specific sequences), and were synthesized at Sangon Bioengineering (Shanghai) Co., Ltd.

[0106] It should be noted that these Oligo sequences cannot be compared (mapped) with the human genome, so they can be used for library quality inspection of human genome sequencing. When it is used for co-sequencing with other species, it needs to be used after targeted analysis.

[0107] The 96 indexes mentioned in the examples of this application are derived from 96 of the 128 indexes in the MGI Easy DNAAdapters-96 (plate format) kit manufactured by...

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Abstract

The invention provides a label quality control method and device. The method comprises the following steps: 1, establishing m libraries for M known sequences, wherein each library contains one or morelabels, each known sequence and each label form a known label-sequence combination, the M libraries include N labels, any two or more libraries at most contain one same label, M and N are natural numbers, and M is less than or equal to N; sequencing the M libraries in the same lane to obtain sequencing original data; and judging whether pollution exists in the N labels or not by analyzing whetherlabel-sequence combinations which do not conform to known label-sequence combinations exist in the sequencing original data or not. According to the method, the number of constructed libraries is small, the number of quality control labels is large, the cost is low, and label low-frequency cross contamination can be found.

Description

technical field [0001] The present invention relates to the technical field of high-throughput sequencing, in particular to a method and device for label quality control. Background technique [0002] DNA sequencing technology has been developed for more than 40 years. From the first-generation DNA sequencing technology (Sanger method) in 1977 to the third-generation and even fourth-generation sequencing technology research today, the read length is from long to short, and then from short to short. Until now, sequencing technology has made leaps and bounds. As far as the current technology is concerned, the second-generation sequencing technology still occupies an absolute dominant position in the global sequencing market, and the third- and fourth-generation sequencing technologies have also developed rapidly in the past two years. Every revolution in sequencing technology also has a huge boost to genome research, disease medical research, drug development, breeding and ot...

Claims

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Application Information

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IPC IPC(8): G16B30/10G16B40/00C12Q1/6869
CPCG16B30/10G16B40/00C12Q1/6869C12Q2535/122Y02P90/30
Inventor 孙广欣王冬石露李占宇杨飘伍启熹王建伟刘倩唐宇
Owner BEIJING USCI MEDICAL LAB CO LTD
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