Unlock instant, AI-driven research and patent intelligence for your innovation.

A kind of cryopreservation solution for female adipose tissue and its preparation method and cryopreservation method

An adipose tissue and cryopreservation technology, applied in the field of biomedicine, can solve the problems of low necrosis and absorption rate, low cell survival rate, adipose tissue toxicity, etc., and achieve good cryopreservation effect, improve cell activity, and promote the effect of proliferation.

Active Publication Date: 2022-05-13
广州市拜沃思生物科技有限公司
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the current formula of this cryopreservation technology is mainly dimethyl sulfoxide (DMSO) and basal culture medium. DMSO is a permeable protective agent that can lower the freezing point of cells, reduce the formation of ice crystals, reduce free radical damage to cells, and change The permeability of biofilms to electrolytes, drugs, poisons and metabolites. DMSO is also a toxic substance. As an antifreeze agent, it can also have a toxic effect on adipose tissue, resulting in low cell survival rate, Necrosis and low absorption rate, infection and other problems

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of cryopreservation solution for female adipose tissue and its preparation method and cryopreservation method
  • A kind of cryopreservation solution for female adipose tissue and its preparation method and cryopreservation method
  • A kind of cryopreservation solution for female adipose tissue and its preparation method and cryopreservation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A female adipose tissue cryopreservation solution is composed of the following substances in parts by weight: 10 parts of dimethyl sulfoxide, 12.5 parts of trehalose, 5 parts of estradiol, and 72.5 parts of DMEM high-sugar medium.

[0029] The preparation method of described female adipose tissue cryopreservation liquid, comprises the following steps:

[0030] (1) Dissolve trehalose in DMEM medium and filter with bacterial filter;

[0031] (2) Add estradiol to dissolve completely and add it into a sterile container;

[0032] (3) Add dimethyl sulfoxide with a pipette and shake well, and store the frozen solution in a refrigerator at 0-6°C.

Embodiment 2

[0034] The difference between Example 2 and Example 1 lies in that the ratio of female adipose tissue cryopreservation solution is different, and the others are the same.

[0035] A female adipose tissue cryopreservation solution is composed of the following substances in parts by weight: 5 parts of dimethyl sulfoxide, 20 parts of trehalose, 2 parts of estradiol, and 80 parts of DMEM high-sugar medium.

Embodiment 3

[0037] The difference between Example 3 and Example 1 lies in that the ratio of female adipose tissue cryopreservation solution is different, and the others are the same.

[0038] A female adipose tissue cryopreservation solution is composed of the following substances in parts by weight: 15 parts of dimethyl sulfoxide, 5 parts of trehalose, 8 parts of estradiol, and 65 parts of DMEM high-sugar medium.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a female adipose tissue cryopreservation solution, which consists of the following substances in parts by weight: 5-15 parts of dimethyl sulfoxide, 5-20 parts of trehalose, 2-8 parts of estradiol, and 65-80 parts of DMEM High glucose medium. The tissue cryopreservation solution of the present invention reduces the toxicity caused by the simple use of DMSO and basal medium to the greatest extent, and ensures a good cryopreservation effect. Estradiol can reduce the cryopreservation damage in adipose tissue cryopreservation, Improve cell viability and freeze storage effect. Estradiol has a great effect on improving the viability of adipose tissue. In the test, the average The cell viability rates were 99.57%, 96.24%, 95.78%, 92.33%, and 90.15%, respectively. The resuscitated tissue can be directly applied to clinical transplantation after clearing the cryopreservation fluid, which is of great value in cosmetic surgery.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a cryopreservation solution for female adipose tissue, a preparation method and a cryopreservation method thereof. Background technique [0002] Autologous fat cosmetology has increasingly become a cosmetic method in cosmetic surgery. Autologous fat cosmetology refers to sucking out excess fat from a certain part of the body through negative pressure suction, and then filling it into other parts of the body. to achieve a specific effect. Autologous fat comes from a wide range of sources. It can be used for one liposuction and multiple times. It can also reduce the pain and financial burden caused by liposuction again. The excess fat extracted can be stored in an ultra-low temperature refrigerator using low-temperature freezing technology. It is time to resuscitate for cosmetic surgery, so the cryopreservation of tissues outside the body becomes extremely important. [0003]...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
CPCA01N1/0221A01N1/0226
Inventor 王键
Owner 广州市拜沃思生物科技有限公司