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A method for microbial in-situ inhibition of hydrogen sulfide release from comprehensive tannery sludge

A technology of microorganisms and hydrogen sulfide, which is applied in the methods of using microorganisms, biochemical equipment and methods, biological sludge treatment, etc. The effect of suppressing the release of hydrogen sulfide

Active Publication Date: 2022-05-13
CHINA LEATHER & FOOTWEAR IND RES INST JINJIANG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Tannery sludge treatment facilities, especially tannery sludge concentration tanks are large-scale open-air facilities. It is necessary to build a collection system to collect hydrogen sulfide before processing, and the investment and maintenance costs are high.

Method used

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  • A method for microbial in-situ inhibition of hydrogen sulfide release from comprehensive tannery sludge
  • A method for microbial in-situ inhibition of hydrogen sulfide release from comprehensive tannery sludge
  • A method for microbial in-situ inhibition of hydrogen sulfide release from comprehensive tannery sludge

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Step 1, preparation of functional microbial flora

[0046] ①Take 0.1L of sludge from a tannery sludge thickening tank in a tannery, and add nutrients to it, which include beef extract and peptone; among them, beef extract is added at a concentration of 2g / L, and peptone is added at a concentration of 4g / L The amount of L was added; culture was shaken at a constant temperature at 30° C. and 100 rpm for 24 hours to obtain a microorganism-enriched culture.

[0047] ② The microbial enrichment culture was inoculated into the sterilized screening medium according to the inoculation amount of 10%, and cultured at a constant temperature and shaking at 30°C and 80rpm for 11 days, and the medium became turbid.

[0048] Wherein, the screening medium is prepared according to the following method: the sludge in the tannery sludge concentration tank is thermally hydrolyzed at 150°C for 10 minutes, then filtered with filter paper, the filtrate is collected, and KH 2 PO 4 2.0g / L, NH ...

Embodiment 2

[0054] Step 1, preparation of functional microbial flora

[0055] ①Take 0.1L of sludge from a tannery sludge thickening tank in a tannery, and add nutrients to it. The nutrients include beef extract and peptone; among them, beef extract is added at a concentration of 5g / L, and peptone is added at a concentration of 10g / L The amount of L was added; culture was shaken at a constant temperature at 25° C. and 60 rpm for 20 hours to obtain a microorganism-enriched culture.

[0056] ② The microbial enrichment culture was inoculated into the sterilized screening medium according to the inoculum amount of 10%, and cultured at a constant temperature and shaking at 30°C and 60rpm for 8 days, and the medium became turbid.

[0057] Wherein, the screening medium is prepared according to the following method: the sludge in the tannery sludge concentration tank is thermally hydrolyzed at 110°C for 15 minutes, then filtered with filter paper, the filtrate is collected, and KH 2 PO 4 2.0g / L...

Embodiment 3

[0063] Step 1, preparation of functional microbial flora

[0064] ①Take 0.1L of sludge from a tannery sludge concentration tank in a tannery, and add nutrients to it, which include beef extract and peptone; among them, beef extract is added at a concentration of 3.5g / L, and peptone is added at a concentration of 7g The amount of / L was added; culture was shaken at a constant temperature at 30°C and 100 rpm for 12 hours to obtain a microorganism-enriched culture.

[0065] ② The microbial enrichment culture was inoculated into the sterilized screening medium according to the inoculum amount of 10%, and cultivated at 30°C and 80rpm for 5 days with constant temperature shaking, and the medium became turbid.

[0066] Wherein, the screening medium is prepared according to the following method: the sludge in the tannery sludge concentration tank is thermally hydrolyzed at 120°C for 10 minutes, then filtered with filter paper, the filtrate is collected, and KH 2 PO 4 2.0g / L, NH 4 ...

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Abstract

The invention discloses a method for microbial in-situ inhibition of hydrogen sulfide release from tannery comprehensive sludge. Firstly, functional microbial flora is prepared: take the sludge in the tannery sludge concentration tank as a sludge sample, and the sludge sample Under the conditions of 25-30°C and 60-100rpm, constant temperature shaking culture for 12-24 hours to obtain the microbial enrichment culture; inoculate the microbial enrichment culture into the sterilized screening medium and cultivate it at 25-30°C, Constant temperature shaking culture at 60-100rpm for 5-11 days; inoculate the cultured screening medium into the sterilized acclimation medium, acclimate to obtain functional microbial flora culture; finally inoculate the functional microbial flora culture Into tannery comprehensive sludge to inhibit the release of hydrogen sulfide. The method can significantly inhibit the release of hydrogen sulfide in tannery sludge; the operation is simple, the cost is low, and the applicability is strong.

Description

technical field [0001] The invention relates to the technical field of tannery sludge treatment, in particular to a method for in-situ microbial inhibition of hydrogen sulfide release from comprehensive tannery sludge. Background technique [0002] Tannery comprehensive sludge contains a large amount of sulfur-containing substances, and hydrogen sulfide is easily produced under the influence of microorganisms and environmental conditions. Being in such an environment for a long time will cause many discomforts to the human body, and even poisoning. Studies have shown that the release of hydrogen sulfide reaches the release equilibrium about 19 hours after the leather comprehensive sludge is discharged, that is, the release of hydrogen sulfide cannot be detected. Therefore, it is the key to solve the hydrogen sulfide pollution in tannery wastewater treatment plants to study the method of quickly removing or inhibiting the release of hydrogen sulfide. [0003] At present, hy...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F11/02C12N1/00C12N1/36
CPCC02F11/02C12N1/00C12N1/36
Inventor 王智张炫辉段徐宾李幸陈娜娜王文琪尹岳涛
Owner CHINA LEATHER & FOOTWEAR IND RES INST JINJIANG CO LTD
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