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Breeding method for kaempferia galanga virus-free seedlings

A technology of virus-free seedlings and kaempferia, which is applied in the field of plant tissue culture, can solve the problems of large commodity kaempferia, high production cost, and consumption, and achieve the effects of shortening the cultivation time, strong tillering ability, and cost saving

Active Publication Date: 2020-04-14
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In conventional cultivation of kaempferia, tuberous stems are used as planting materials; when harvesting, rhizomes that are healthy, free from diseases and insect pests, and not affected by freezing are selected, and they are stored in sand for overwintering as seedlings; this not only consumes a lot of commercial kaempferia, but also requires a lot The land is reserved for seeds, the production cost is high, and there are problems such as serious pests and diseases
Especially for disasters caused by viral diseases, there is no specific medicine for prevention and treatment at present

Method used

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  • Breeding method for kaempferia galanga virus-free seedlings
  • Breeding method for kaempferia galanga virus-free seedlings
  • Breeding method for kaempferia galanga virus-free seedlings

Examples

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Effect test

Embodiment 1

[0042] The breeding method of the kaempferia virus-free seedling of the present embodiment may further comprise the steps:

[0043] (1) Explant preparation: take the vigorously growing underground tubers of Kaempferia in that year, cut off the roots on the tubers, wash off the soil on the surface with running water, gently brush the surface with a hair brush, dry, and remove the buds. Cut it down to about 1.0cm in diameter, use a scalpel to gently scrape off the epidermis and the surrounding brown tissue, especially near the base of the buds, be careful not to hurt the young buds, until the epidermis and leaf sheaths are completely peeled off, Complete explant preparation; then place in sterile water for later use.

[0044] (2) Aseptic buds are obtained: the explants are rinsed twice with sterile water on an ultra-clean workbench, and then sterilized with 75% ethanol aqueous solution by volume fraction (keep shaking during the disinfection process to make it fully sterilized; ...

Embodiment 2

[0051] The breeding method of the kaempferia virus-free seedling of the present embodiment may further comprise the steps:

[0052] (1) Explant preparation: take the vigorously growing underground tubers of Kaempferia in that year, cut off the roots on the tubers, wash off the soil on the surface with running water, gently brush the surface with a hair brush, dry, and remove the buds. Cut it down to about 1.0cm in diameter, use a scalpel to gently scrape off the epidermis and the surrounding brown tissue, especially near the base of the buds, be careful not to hurt the young buds, until the epidermis and leaf sheaths are completely peeled off, Complete explant preparation; then place in sterile water for later use.

[0053] (2) Aseptic buds are obtained: the explants are rinsed twice with sterile water on an ultra-clean workbench, and then sterilized with 75% ethanol aqueous solution by volume fraction (keep shaking during the disinfection process to make it fully sterilized; ...

Embodiment 3

[0060] The breeding method of the kaempferia virus-free seedling of the present embodiment may further comprise the steps:

[0061] (1) Explant preparation: take the vigorously growing underground tubers of Kaempferia in that year, cut off the roots on the tubers, wash off the soil on the surface with running water, gently brush the surface with a hair brush, dry, and remove the buds. Cut it down to about 0.5cm in diameter, use a scalpel to gently scrape off the epidermis and the surrounding brown tissue, especially the part near the base of the bud, be careful not to hurt the young bud, until the epidermis and leaf sheath are completely peeled off, Complete explant preparation; then place in sterile water for later use.

[0062] (2) Aseptic buds are obtained: the explants are rinsed twice with sterile water on an ultra-clean workbench, and then sterilized with 70% ethanol aqueous solution by volume fraction (keep shaking during the disinfection process to make it fully steril...

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Abstract

The invention discloses a breeding method for kaempferia galanga virus-free seedlings. The method disclosed by the invention comprises the following steps: preparing explants, obtaining sterile buds,obtaining subculture strong buds of the sterile buds, performing multiplication and rooting of sterile seedlings, and strengthening and transplanting rooting seedlings, namely when the rooting seedlings grow to 6-8cm high, performing room-temperature seedling strengthening, moving the seedlings to a greenhouse, and finally transplanting the seedlings into a field. A multiplication coefficient of abud obtained by using the method disclosed by the invention is 3.27-5.2, a rooting rate of obtained tissue culture seedlings is 100%, a survival rate of transplanting is 100%, infections of diseasesand insects are avoided, and the problem that disease and insect infections can be easily caused in a conventional planting mode of the kaempferia galanga can be effectively solved.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for breeding virus-free seedlings of kaempferia. Background technique [0002] Kaempferia galanga L., also known as sand ginger, mountain pepper, etc., belongs to the perennial root herb of the genus Kaempferia in the family Zingiberaceae. It is drought-tolerant, barren-resistant and afraid of soaking. It is distributed and cultivated in Guangdong, Guangxi, Yunnan, Taiwan and other provinces. The medicinal or edible parts of kaempferia are underground tuberous stems, solitary or connected in several pieces, light green or greenish white, and fragrant; kaempferia is not only a famous traditional Chinese medicine, but also an edible condiment with unique flavor, and also a food processing spice, Raw materials such as soy sauce are very popular in the market. [0003] In conventional cultivation of kaempferia, tuberous stems are used as planting ma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 陈娟王佳佳夏念和
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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