Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Primers and probes for rapid detection and identification of cryptococcus and their application

A technology of cryptococcus and cryptococcus neoformans, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of cumbersome operation, high error rate, low sensitivity, etc., and achieve simple operation, strong applicability, The effect of high sensitivity

Active Publication Date: 2020-07-03
北京量觉科技有限责任公司
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the defects of tedious operation, low sensitivity and high error rate of the traditional method for detecting Cryptococcus neoformans and Cryptococcus gattii, the present invention provides a primer and probe for rapid detection and identification of Cryptococcus and its application

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Primers and probes for rapid detection and identification of cryptococcus and their application
  • Primers and probes for rapid detection and identification of cryptococcus and their application
  • Primers and probes for rapid detection and identification of cryptococcus and their application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Target genes, primers, probes and kits for detection and identification of Cryptococcus neoformans and Cryptococcus gattii

[0028] Dozens of genome sequences of Cryptococcus neoformans and Cryptococcus gattii were obtained through high-throughput sequencing technology, and the genome sequence differences of dozens of Cryptococcus were compared by genomics methods, and CAP59 was finally selected through preliminary primer and probe design The sequence is used as the target gene, and the gene fragment is relatively conserved in the two Cryptococcus species, and there are certain differences between Cryptococcus neoformans and Cryptococcus gattii. The nucleotide sequences of target genes for detecting Cryptococcus neoformans and Cryptococcus gattii are shown in SEQ ID NO:5 and SEQ ID NO:6.

[0029] Design primers for the selected target gene sequence information. Through repeated comparative analysis, real-time fluorescent PCR detection primers and probes that can be use...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the field of fungal etiology detection, and discloses primers and probes for detecting Cryptococcus neoformans and Cryptococcus gattii; the probes include detecting Cryptococcus neoformans and Probes for Cryptococcus gattii. The present invention puts the raw materials required for detecting Cryptococcus neoformans and Cryptococcus gattii in the same reaction system, can simultaneously detect and identify Cryptococcus neoformans and Cryptococcus gattii, clarify the types of pathogenic bacteria, and help fast and accurate clinical diagnosis, Gain precious time for disease treatment.

Description

technical field [0001] The invention belongs to the field of fungal etiology detection, in particular to primers and probes for rapid detection and identification of cryptococcus and applications thereof. Background technique [0002] In fungal taxonomy, Cryptococcus belongs to Deuteromycotina, Bacillus, Cryptococcus, and Cryptococcus. There are many species in Cryptococcus, which are widely distributed in nature. Cryptococcus neoformans and Cryptococcus gattii. In recent years, cryptococcal infection is on the rise at home and abroad. [0003] Because cryptococcal infection is easily misdiagnosed based on clinical manifestations, etiological detection is the main basis for diagnosis and treatment. At present, the main cryptococcus detection methods have limitations. Among them, cerebrospinal fluid ink staining can initially observe the morphology of cryptococcus, but the sensitivity is low; Cryptococcus special; Cryptococcus culture identification is also difficult to id...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12Q1/686C12Q1/04C12N15/11C12R1/645
CPCC12Q1/686C12Q1/6895C12Q2563/107
Inventor 不公告发明人
Owner 北京量觉科技有限责任公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com