Preparation and antifouling evaluation method of metal surface modified by extracting cyclopeptide from traditional Chinese medicine
A metal surface and cyclic peptide technology, which is applied in the field of metal surface preparation and antifouling evaluation, can solve problems such as biofouling on the surface of marine metal materials, and achieve the effects of easy availability of raw materials and simple operation.
Active Publication Date: 2022-01-11
YANGZHOU UNIV
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[0003] The object of the present invention is to, in order to overcome the defect in the prior art, provide a kind of preparation and the antifouling evaluation method of the metal surface of the cyclic peptide modification of Chinese medicine extraction, solve the technical problem of biofouling on the surface of marine metal materials in the prior art , using the present invention to modify the metal surface can improve the antifouling performance
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[0032] The present invention will be further described below in conjunction with the accompanying drawings and implementation methods.
[0033] Such as figure 1 A method for preparing a metal surface modified by extracting cyclic peptides from traditional Chinese medicines shown, comprising the following steps:
[0034] (1) Dried and crushed into powder;
[0035] (2) According to the principle of "similar polarity and compatibility" between the chemical components of natural products and solvents, the antibacterial cyclic peptide in 1000 g of violadin powder was extracted with a mixed solution of 10L ethanol and water, and assisted by microwaves, ethanol and water The volume ratio is 3:2, the microwave-assisted time is 0.5-3h, and the temperature is kept at 20±2°C;
[0036] (3) Realize solid-liquid separation of the solution containing violadin by high-speed centrifugation to obtain a solution containing antibacterial cyclic peptide;
[0037] (4) Use high performance liquid...
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The invention discloses a preparation and antifouling evaluation method of a metal surface modified by extracting cyclopeptide from a traditional Chinese medicine in the technical field of metal material preparation, comprising the following steps: (1) drying and crushing Viola chinensis into powder; 2) Using a mixed solution of ethanol and water to extract the antimicrobial cyclic peptides in 1000 g of violadin powder, and assisted by microwave; (3) The solution containing violadin was separated from solid to liquid by high-speed centrifugation to obtain the antibacterial cyclic peptides containing antibacterial cyclic peptides. Peptide solution; (4) Use high performance liquid chromatography to separate and purify the antibacterial cyclic peptide; (5) Use tris(2-carboxyethyl)phosphine and trypsin to reduce and digest the cyclic peptide; (6) Choose 304 stainless steel as the substrate , polish it to the mirror surface, wash and dry it and soak it in dopamine solution for 24 hours; (7) immerse the activated stainless steel surface in the solution of antibacterial cyclopeptide for 24 hours, take out the sample, and dry it under nitrogen environment; use the present invention to modify metal The surface can improve the antifouling performance.
Description
technical field [0001] The invention belongs to the technical field of metal material preparation, and in particular relates to a preparation and antifouling evaluation method of a metal surface modified by extracting cyclopeptide from a traditional Chinese medicine. Background technique [0002] When the hull is immersed in seawater, its surface will be quickly attached by bacteria or other microorganisms, causing fouling on the surface of the hull. Biofouling is a serious problem faced by the global shipping industry. It reduces the maneuverability of ships and improves operational efficiency. Cost, the coatings commonly used for hulls are harmful to the surrounding environment and are not easy to protect the marine environment. Contents of the invention [0003] The object of the present invention is to, in order to overcome the defect in the prior art, provide a kind of preparation and the antifouling evaluation method of the metal surface of the cyclic peptide modific...
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IPC IPC(8): C07K1/14C12R1/63C12R1/07C12R1/89C12R1/01
CPCC23F11/145C07K1/145C12P21/06C12Q1/02G01N21/64G01N2333/28G01N2333/24G01N2333/32G01N2333/405
Inventor 曹攀刘可为尹彬沣吴多利张超
Owner YANGZHOU UNIV