Immunoturbidimetry apolipoprotein B single reagent with stable liquid and freezing resistance

An immunoturbidimetric and apolipoprotein technology, applied in the field of medical testing, can solve problems that do not involve liquid single reagents

Pending Publication Date: 2020-04-17
桂林英美特生物技术研究所
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  • Abstract
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  • Claims
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Problems solved by technology

[0006] The prior art does not involve the formulation of liquid single reagents, mainly because it fails to solve the stability problem of the coexistence of specific antibodies and non-specific polyethylene glycol sensitizers in single reagents

Method used

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  • Immunoturbidimetry apolipoprotein B single reagent with stable liquid and freezing resistance

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Embodiment Construction

[0014] The following describes the embodiment of the present invention in detail. The antibody treatment solution includes a buffer solution with a pH value of 7.0-8.0 of 20-200mmol / L, polyethylene glycol 2000-1000030.0-80.0g / L, and magnesium salt 5.0-30.0g / L. L. Preservatives 0.2-2.0g / L. The buffer includes phosphate buffered saline (PBS), N-2-hydroxyethylpiperazine-N-2-ethanesulfonic acid buffer (HEPES) or Tris-hydrochloric acid (Tris- HCL) buffer solution, adding the buffer solution to the antibody treatment solution can provide stable ionic strength and pH value for antibody pre-precipitation. The polyethylene glycol 2000-10000 includes one of polyethylene glycol 2000, polyethylene glycol 4000, polyethylene glycol 6000, polyethylene glycol 8000, and polyethylene glycol 10000. In the antibody treatment solution Adding the polyethylene glycol 2000-10000 in the antibody can accelerate the precipitation of related proteins in the antibody. The magnesium salt includes one of ...

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Abstract

The invention discloses an immunoturbidimetry apolipoprotein B single reagent with stable liquid and freezing resistance. The single reagent comprises 20-200mmol / L Buffer solution with a PH value ranging from 7.0 to 8.0 , 40.0-200.0 g / L of ApoB antibody subjected to antibody treatment liquid pre-precipitation and centrifugal treatment, 5.0-50.0 g / L of polyethylene glycol 2,000-10,000, 20.0-200.0 g / L of glycerol, 0.5-5.0 g / L of surfactant, and 0.2-2.0 g / L of preservative, wherein the antibody treating fluid comprises 20-200 mmol / L of buffer solution with the pH value ranging from 7.0 to 8.0, 30.0-80.0 g / L of polyethylene glycol 2,000 to 10,000, 5.0-30.0 g / L of magnesium salt and 0.2-2.0 g / L of preservative. Under low temperature conditions, under the promotion action of polyethylene glycoland magnesium salt with a certain concentration, protein easy to precipitate in the antibody is precipitated in an accelerated mode, then high-speed centrifugation is conducted, precipitate is removed, stability is improved when the antibody and the non-specific polyethylene glycol sensitizer coexist in the single reagent, protein precipitation after the reagent is frozen is prevented, and therebyperformance of the reagent is prevented from being affected.

Description

technical field [0001] The invention relates to the technical field of medical detection, in particular to a liquid-stable, anti-freeze immunoturbidimetric apolipoprotein B single reagent. Background technique [0002] Apolipoprotein B (ApoB) is mainly synthesized in the liver. It is the main structural protein of low-density lipoprotein (LDL) and acts as a ligand for LDL receptors, which can regulate the clearance rate of LDL from plasma. The measured value of ApoB in blood directly reflects the content of LDL. [0003] The clinical significance of the level of ApoB is similar to that of low-density lipoprotein cholesterol (LDL-C), and it is a better marker of atherosclerosis among various blood lipid indicators. In a few cases, high ApoB levels may occur but LDL-C is normal, suggesting that there are many small and dense LDLs in the blood, and it is more advantageous to measure ApoB. In the drug intervention experiments on patients with coronary heart disease with high A...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/92G01N33/531
CPCG01N33/92G01N33/531G01N2333/775
Inventor 刘红平肖佳民
Owner 桂林英美特生物技术研究所
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