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Rapid detection method for swine fever

A detection method and fast technology, applied in the direction of measuring devices, disease resistance to media transmission, material excitation analysis, etc., can solve the problem of easy to produce more errors in detection, and achieve the elimination of interference from fat and protein impurities, small errors, and detection fast effect

Active Publication Date: 2020-04-21
安徽云燕食品科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In view of the problem in the prior art that there are a small amount of fat and protein impurities in the sample during the detection process, which causes more errors in the detection, the first purpose of the present invention is to provide a rapid detection method for swine fever to solve the above-mentioned technical problems. It can effectively eliminate the interference of fat and protein impurities in the detection process, and has the advantages of fast detection speed, high accuracy and small error

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  • Rapid detection method for swine fever

Examples

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Effect test

Embodiment 1

[0037] Embodiment 1: a kind of rapid detection method of swine fever, prepares and obtains by following steps:

[0038] Step 1, crushing and grinding: take the porcine tissue sample to be tested, shred it fully and grind it in a milk pot, then add 6.5 times the volume of PBS solution, stir and mix to obtain an emulsion;

[0039] Step 2, centrifugal filtration: move the emulsion into a centrifuge tube for centrifugation, the centrifugation speed is 13000rpm, the centrifugation time is 9min, and the centrifugation temperature is set at 3.5°C, then take the supernatant in the centrifuge tube and filter it with a filter with a pore size of 0.22μm Obtain sterile filtrate after membrane sterilization;

[0040] Step 3, Precipitation and impurity removal: Add the sterile filtrate into the container, and add a precipitation aid mixed with trichloroacetic acid, zinc acetate, and potassium ferrocyanide, ultrasonically oscillate, stand to separate and separate, remove the precipitate, and p...

Embodiment 2

[0045] Embodiment 2: a kind of rapid detection method of swine fever, the difference with embodiment 1 is, specifically comprises the following steps:

[0046] Step 1, crushing and grinding: take the porcine tissue sample to be tested, fully cut it into pieces, put it in a milk pot and grind it, then add 5 times the volume of PBS solution, stir and mix to obtain an emulsion;

[0047] Step 2, centrifugal filtration: move the emulsion into a centrifuge tube for centrifugation, the centrifugation speed is 12000rpm, the centrifugation time is 10min, and the centrifugation temperature is set at 4°C, then take the supernatant in the centrifuge tube and filter it with a filter with a pore size of 0.2μm Obtain sterile filtrate after membrane sterilization;

[0048] Step 3, Precipitation and impurity removal: Add the sterile filtrate into the container, and add a precipitation aid mixed with trichloroacetic acid, zinc acetate, and potassium ferrocyanide, ultrasonically oscillate, stand...

Embodiment 3

[0052] Embodiment 3: a kind of rapid detection method of swine fever, the difference with embodiment 1 is, specifically comprises the following steps:

[0053] Step 1, crushing and grinding: take the porcine tissue sample to be tested, fully cut it into pieces, put it in a milk pot and grind it, then add 8 times the volume of PBS solution, stir and mix to obtain an emulsion;

[0054] Step 2, centrifugal filtration: move the emulsion into a centrifuge tube for centrifugation, the centrifugation speed is 14000rpm, the centrifugation time is 8min, and the centrifugation temperature is set at 3°C, then take the supernatant in the centrifuge tube and filter it with a filter with a pore size of 0.24μm Obtain sterile filtrate after membrane sterilization;

[0055] Step 3, Precipitation and impurity removal: Add the sterile filtrate into the container, and add a precipitation aid mixed with trichloroacetic acid, zinc acetate, and potassium ferrocyanide, ultrasonically oscillate, stand...

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Abstract

The invention, which relates to the technical field of poultry hazard detection, discloses a rapid detection method for swine fever, thereby solving the problem that a small amount of fat and proteinimpurities exist in a sample in the detection process, so that more errors are easily generated in detection. The rapid detection method comprises the following steps: step 1, carrying out crushing and grinding; step 2, carrying out centrifugal filtration; step 3, carrying out precipitation and impurity removal; step 4, carrying out inoculation culture; step 5, carrying out fluorescent antibody staining; and step 6, carrying out observing and comparing. In the actual application process, the rapid detection method for the swine fever can be used for effectively eliminating the interference offat and protein impurities in the detection process and has the advantages of being high in detection speed, high in accuracy and small in error.

Description

technical field [0001] The invention relates to the technical field of poultry hazard detection, more specifically, it relates to a rapid detection method for swine fever. Background technique [0002] Introduction Classical swine fever, commonly known as "rotten intestinal fever", is an acute, febrile, contagious infectious disease caused by the swine fever virus of the family Flaviviridae, which is highly contagious and fatal. Under certain conditions, only pigs are infected. Pigs and wild boars of different ages, sexes, and varieties are susceptible, and can occur throughout the year. [0003] In the Chinese invention patent application document with publication number CN108441581A, a method for detecting CSFV is disclosed, which can effectively detect the distribution of CSFV pathogens in swine herds, and the primer set used for detecting CSFV, Described primer set comprises the primer pair that is used for the first amplification, and the sequence of its upstream prime...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/6458G01N21/6486Y02A50/30
Inventor 戈吴超王佳
Owner 安徽云燕食品科技有限公司
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