Parallel confocal detector based on Darman raster
A Damman grating and detection device technology, applied in measuring devices, material analysis through optical means, fluorescence/phosphorescence, etc., can solve the problem of poor focus spot quality and detection lateral resolution, relatively high requirements for light source uniformity, and difficult Processing microlens arrays and other issues to achieve the effect of short data collection time, conducive to fluorescence detection, and easy production
Inactive Publication Date: 2003-06-04
TSINGHUA UNIV
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[0004] The disadvantages of the microarray three-dimensional surface parallel confocal detection device are: 1. Due to the limitation of the process, it is difficult to process a microlens array with a large numerical aperture, and the quality of the focal spot and the lateral resolution of the detection are poor; 2. The microlens array The array has relatively high requirements on the uniformity of the light source, otherwise the focal light intensity of each microlens in the microlens array will be uneven, which will cause large measurement errors; 3. The spacing of the light source lattice formed by the micro-optical array synthesis device is fixed It cannot be adjusted. In the process of three-dimensional imaging, the lattice spacing of each layer detected is different; 4. There is no corresponding small hole filter array before the detector, which is not a complete confocal system, eliminating Stray light is less effective
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[0020] Below in conjunction with accompanying drawing, introduce the content of the present invention in detail.
[0021] The concept of direction description is used in the following description: let the light source be located at the leftmost end of the entire system diagram, the light propagation direction from the light source is right, and the straight line passing through the center of the light source from left to right is defined as the horizontal optical axis. The sample is located at the bottom of the entire system diagram, the detector is located at the top of the system diagram, and the straight line passing through the respective centers of the sample and the detector is defined as the vertical optical axis. Wherein, the horizontal optical axis and the vertical optical axis only represent the relative positions in the figure, and do not indicate the horizontal and vertical physical directions in practical applications. The system can make the plane defined by the ...
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A parallel confocal detecter based on Darman raster is composed of light source, pinhole filter, light-gathering lens, Darman raster, bidirectional chromatic mirror, imaging lens, filter, confocal pinhole array plate and 2D photoelectric detector. The specimen to be detected is put under the bidirectional chromatic mirror. Its advantages are uniform light intensity among more channels, adjustable distance between channels, high effect on eliminating stray light beams, simple structure and low cost.
Description
technical field [0001] The invention relates to a parallel confocal detection device based on a Damman grating, which can be applied to biological research, biomedical research, material science, and industrial product manufacturing and processing detection, and belongs to the technical field of optical imaging and detection devices. Background technique [0002] The traditional confocal laser scanning microscope is a single-point detection, which must be scanned for a long time to produce a two-dimensional image. The quality of the focal spot and the lateral resolution of the detection are poor, the intensity of the light is not uniform, the spacing of the light source lattice cannot be adjusted, the spacing of the lattice of each layer of the three-dimensional detection is different, and the effect of eliminating stray light is poor. [0003] The latest existing parallel confocal detection device is a microarray three-dimensional surface parallel confocal detection device ...
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IPC IPC(8): G01J3/18G01N21/17G01N21/64
Inventor 邬敏贤杨蓉何庆声严瑛白金国藩
Owner TSINGHUA UNIV