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Puffer fish ACE inhibitory peptide and preparation method thereof

A technology of inhibitory peptide and inhibition rate, which is applied in the field of fugu ACE inhibitory peptide and its preparation, to achieve the effect of improving inhibition and high stability

Active Publication Date: 2020-04-28
FISHERIES RES INST OF FUJIAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] There are existing technologies for preparing ACE-inhibiting peptides from hazelnuts, yak milk, shellfish, wolfberry and other foods, but there is no technology for preparing ACE-inhibiting peptides from puffer fish, especially puffer fish skin as raw materials

Method used

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  • Puffer fish ACE inhibitory peptide and preparation method thereof
  • Puffer fish ACE inhibitory peptide and preparation method thereof
  • Puffer fish ACE inhibitory peptide and preparation method thereof

Examples

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Embodiment Construction

[0034] The present invention will be further described in conjunction with the accompanying drawings and specific embodiments.

[0035] The puffer fish ACE inhibitory peptide provided in this example is a nonapeptide, and its amino acid sequence is: GDRGFPGER (Gly-Asp-Arg-Gly-Phe-Pro-Gly-Glu-Arg).

[0036] The molecular weight of nonapeptide GDRGFPGER (Gly-Asp-Arg-Gly-Phe-Pro-Gly-Glu-Arg) is 989.5Da.

[0037] This embodiment also provides a preparation method of puffer fish ACE inhibitory peptide, comprising the following steps:

[0038]S1: Preparation of puffer fish enzymatic hydrolyzate, after beating 100g puffer fish skin, add 300mL distilled water and stir to form protein solution, adjust pH value to 8.0 with NaOH, then add alkaline protease, the enzyme amount is 500U / g, Enzyme hydrolyze at 55°C for 10 hours, keep the pH value unchanged, heat at 90°C for 15 minutes after enzymolysis, then cool to room temperature, adjust the pH value to neutral with HCl, then use 5000r / mi...

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Abstract

The invention discloses a puffer fish ACE inhibitory peptide and a preparation method thereof, and belongs to the technical field of ACE inhibitory peptides, and the preparation method of the puffer fish ACE inhibitory peptide comprises the following steps: S1, preparation of puffer fish enzymatic hydrolysate, S2, ultrafiltration separation of the puffer fish enzymatic hydrolysate, S3, separationand purification of polypeptides, S4, sequence identification of the polypeptides, and S5, polypeptide synthesis and activity determination. The invention relates to a puffer fish ACEinhibitory peptide, which is nonapeptide and has an amino acid sequence of GDRGFPGER (Gly-Asp-Arg-Gly-Phe-Pro-Gly-Glu-Arg). According to the puffer fish ACE inhibitory peptide and the preparation method thereof, puffer fish skin is adopted as a raw material, a nonapeptide amino acid sequence is obtained, and the prepared polypeptide has ACE inhibitory activity.

Description

technical field [0001] The invention belongs to the technical field of ACE inhibitory peptides, and in particular relates to a puffer fish ACE inhibitory peptide and a preparation method thereof. Background technique [0002] With the progress and development of society, people's living standards are gradually improving, and the proportion of fat and calories in the diet is gradually increasing. Coupled with the reduction in exercise, the incidence of cardiovascular diseases in my country has risen rapidly, of which hypertension is the main one. [0003] Pufferfish is a bottom fish in the warm temperate zone and tropical coastal waters. It inhabits the middle and lower layers of the ocean. A few species enter freshwater rivers. When encountering external danger, the whole body floats to the surface in a spherical shape, and at the same time, the small spines on the skin stand up. , for self-defense. [0004] Angiotensin-converting enzyme (ACE) inhibitory peptide is a small ...

Claims

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Application Information

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IPC IPC(8): C07K7/06C07K1/36C07K1/34C07K1/16C12P21/06A61P9/12
CPCA61P9/12C07K7/06C12P21/06
Inventor 苏永昌刘智禹刘淑集陈贝乔琨许旻陈晓婷
Owner FISHERIES RES INST OF FUJIAN
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