Salmonella abortus equi horse derived strain and application thereof in preparation of salmonella equina inactivated vaccine

A technology of salmonella and inactivated vaccines, which is applied in the direction of vaccines, veterinary vaccines, bacteria, etc., can solve the problems of spreading, affecting the stock of horses, indirect economic losses, etc.

Active Publication Date: 2020-05-05
HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The occurrence of the disease has directly affected the stock of horses in my country and caused serious indirect economic losses. Moreover, the disease has a tendency to spread, which has a potential threat and a huge impact on the sustainable development of my country's horse industry, seriously hindering the development of my country's horse industry. industry development

Method used

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  • Salmonella abortus equi horse derived strain and application thereof in preparation of salmonella equina inactivated vaccine
  • Salmonella abortus equi horse derived strain and application thereof in preparation of salmonella equina inactivated vaccine
  • Salmonella abortus equi horse derived strain and application thereof in preparation of salmonella equina inactivated vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Isolation and culture identification of Salmonella equine abortus strain 20180316.H.AES.G

[0033] According to the new biological product declaration requirement, in conjunction with a large amount of test data that the present invention obtains, with reference to " Chinese Veterinary Pharmacopoeia " (2005 edition), the poisonous species used for making seedlings has been identified, and the poisoned species standard of the trial procedure (draft) is now carried out as follows illustrate.

[0034] 1 Isolation and identification of virus species

[0035] The virus species used to manufacture this product is Salmonella equine abortifaciens equine source strain 20180316.H.AES.G, which was isolated from an aborted foal tissue by the inventor of the present invention in 2018 from a horse farm in Hulunbeier City, Inner Mongolia. Salmonella abortus.

[0036] The liver tissue of the aborted foal was streak-inoculated on the Salmonella chromogenic medium, and the Sa...

Embodiment 2

[0052] The preparation of embodiment 2 inactivated vaccines

[0053] 1 inactivation process

[0054] Choose formaldehyde solutions with final concentrations of 0.1% v / v, 0.15% v / v, and 0.2% v / v to inactivate at 4, 28°C, and 37°C, and inactivate 0.2% formaldehyde at 28°C for 48 hours, and the inactivation effect is complete . Therefore, it is finally determined that 0.2% v / v formaldehyde is inactivated at 28°C for 48 hours, coated with no anti-LB plate, cultured at 37°C for 3 days, and sterile, then the inactivation is qualified, and then the inactivation is continued for 12 hours. Wash 2 times with PBS to remove formaldehyde.

[0055] 2 emulsification process

[0056] Commercialized MONTANIDE ISA35 adjuvant from Sebec, France, and 0.2% v / v formaldehyde (HCHO) inactivated Salmonella equine abortus strain 20180316.H.AES.G were mixed and emulsified to make inactivated vaccine, adjuvant The concentration is 25%. Make up to volume with PBS.

[0057] 3 Quality standards for in...

Embodiment 3

[0078] Embodiment 3 horse safety test

[0079] 3 batches of inactivated vaccines qualified by the safety test prepared in Example 2 were immunized to stallions, non-pregnant and pre-pregnancy, mid-pregnancy, late-pregnancy mares (10 billion / ml / horse, 1.0 × 10 10 CFU / ml), observe whether the experimental horse absorbs the vaccine, whether the diet, drinking water, and mental state are normal. The results showed that only a few horses did not like to eat grass, drinking water was normal, and some horses had swelling at the immune site, but no ulceration. Generally, the swelling subsided on the 4th day, and the swelling disappeared completely within 2 weeks. Individual body temperature rises briefly and returns to normal within 2 days. After one month of observation, vaccine immunization will not cause abortion in pregnant mares, as shown in Table 5.

[0080] Table 5 Horse body safety test

[0081]

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Abstract

The invention discloses a salmonella abortus equi horse derived strain and an application thereof in preparation of a salmonella equina inactivated vaccine, and belongs to the technical field of biological vaccines. The salmonella abortus equi horse derived strain is named as 20180316.H.AES.G and is preserved in the common microorganism center of the horse China General Microbiological Culture Collection Center, wherein the culture collection number is CGMCC No.18341. The salmonella abortus equi horse derived strain 20180316.H.AES.G provided by the invention has the advantages of good immunogenicity, good culture characteristics and the like. An inactivated vaccine is prepared by the steps of inoculating the salmonella abortus equi horse derived strain 20180316.H.AES.G to a salmonella equienrichment broth, performing culturing, and then performing inactivating with 0.2% v / v formaldehyde. Experiments prove that when a mouse is immunized by the prepared inactivated vaccine of the salmonella abortus equi, the mouse can be free from death after challenge, and safety is good after a horse body is immunized. Therefore, the vaccine has the advantages of safety, low-dose immunization andthe like. The invention provides the inactivated vaccine of salmonella abortus equi for the first time, and the inactivated vaccine can be used for preventing abortion caused by salmonella abortus equi.

Description

technical field [0001] The present invention relates to the application of a Salmonella equine abortus in the preparation of an inactivated vaccine of Salmonella equine abortus, in particular to a self-isolated Salmonella equine abortus equine source strain and the inactivated vaccine prepared by the bacterial strain. The invention belongs to the field of biological vaccines. Background technique [0002] Equine abortus salmonellosis, also known as equine paratyphoid abortion (Equi parathphoid), is an infectious disease characterized by abortion in equine animals caused by Salmonella abortus equi. Animals are pathogenic, with bacterial antigens 4 and 12, and flagellar second-phase antigen H-enx. As early as the end of the 18th century and the beginning of the 19th century, a large number of horse abortions occurred in Europe and the United States. In the late 1970s, horse breeding areas such as North China, Northwest, and Northeast China also broke out in succession. Aborti...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K39/112A61P31/04A61P15/06C12R1/42
CPCA61K39/0275A61K2039/521A61K2039/552A61P15/06A61P31/04C12N1/205C12R2001/42
Inventor 王晓钧胡哲郭巍刘荻萩
Owner HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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