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A method for enzymatic hydrolysis and saccharification of lignocellulose

A lignocellulosic enzyme and lignocellulose technology, which is applied in the field of lignocellulose enzymatic hydrolysis and saccharification, can solve the problems of low fermentable sugar concentration and low enzymatic hydrolysis efficiency, reduce ineffective adsorption, use less additives, and promote The effect of enzymatic hydrolysis

Active Publication Date: 2021-08-06
CHINA PETROLEUM & CHEM CORP +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Aiming at the problems of low enzymatic hydrolysis efficiency and low fermentable sugar concentration existing in the prior art, the present invention provides a method for enzymatic hydrolysis and saccharification of lignocellulose

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 (n=9)

[0031] Synthesis of CH by microwave-assisted Fmoc solid-phase method 3 CO-A 9 K-NH 2 , the specific steps are:

[0032] (1) Deprotection: Use 20% piperidine / DMF solution to remove the Fmoc protecting group 9-fluorenylmethoxycarbonyl of the amino group on the Rink amide-MBHA resin.

[0033] (2) Activation: Use an activator to activate the carboxyl group of lysine. The activator is HOBT and HBTU (ratio 1:1), DMF is the solvent, and the concentration is 0.45mol / L.

[0034] (3) Cross-linking: After the carboxyl group of lysine is activated, it undergoes an acylation reaction with the free amino group in the presence of microwave-assisted heating and an activated base to form a peptide bond. The activated base is DIEA, DMF is the solvent, the concentration is 2mol / L, and the reaction temperature is 75°C.

[0035] (4) Synthesis: Repeat steps (1)-(3) to connect all alanines in the sequence to the resin to obtain a solid-phase carrier with the target pol...

Embodiment 2

[0040] Same as Example 1, the difference is: take the polypeptide CH prepared in Example 1 3 CO-A 9 K-NH 2 Dissolve it in a citric acid-sodium citrate buffer solution with a pH of 5, the concentration is 5mmol / L, and then add it in an amount of 0.25% of the total mass of the enzymatic hydrolysis system, and the saccharification rate is 82.43%.

Embodiment 3

[0042] With embodiment 1, difference is: the CH synthesized by microwave-assisted Fmoc solid-phase method 3 CO-A 8 K-NH 2 , The saccharification rate is 79.01%.

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Abstract

The invention relates to a method for enzymatic saccharification of lignocellulose, which comprises adding a polypeptide during the enzymatic hydrolysis of lignocellulose, and the sequence of the polypeptide is CH 3 CO‑A n K‑NH 2 (3≤n≤9), A stands for alanine, K stands for lysine. The invention adopts the polypeptide as an enzymolysis auxiliary agent, improves the enzymolysis efficiency and fermentable sugar concentration, and has the characteristics of high saccharification rate and less auxiliary agent usage.

Description

technical field [0001] The invention belongs to the technical field of biomass energy, and in particular relates to a method for enzymatic hydrolysis and saccharification of lignocellulose. Background technique [0002] As a rich and cheap renewable resource, cellulose is one of the most abundant biomass in nature, accounting for 50% of the total biomass in the world, and the dry matter produced by photosynthesis of green plants around the world can reach 1.55×10 per year. 11 Tons, including agricultural and forestry waste, waste paper, energy crops, etc., most of which are currently unused and discarded, of which the carbohydrate content is as high as 75%, which can be used as an important source of fermentable sugars to produce liquid fuels and other chemicals. With the shortage of petroleum resources and people's increasing attention to energy and environmental issues, using cellulose biomass as raw material and converting it into biomass energy that can replace fossil en...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/14
CPCC12P19/14
Inventor 王蒙张全高慧鹏关浩
Owner CHINA PETROLEUM & CHEM CORP
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