Injectable colored gel pad for gastrointestinal mucosa delamination and application thereof.

A color gel pad and digestive tract technology, applied in the field of biomedical engineering, can solve problems such as unscientific use, and achieve the effects of convenient peeling, high biocompatibility, convenient and safe mucosa peeling

Active Publication Date: 2020-05-08
NANTONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although methylene blue can be used as a chemical indicator, dye, biological stain and medicine, it is often used clinically to treat urinary calculi, obliterative vasculitis, and neurodermatitis, but the International Agency for Research on Cancer of the World Health Organization lists methylene blue in 3 In the list of carcinoids, it is unscientific to use it clinically as a biological chromogenic agent

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Preparation of genipin solution, silk fibroin solution, chitosan solution and gelatin solution.

[0031] Preparation of genipin solution: Dissolve 10 g of genipin and 6 g of disodium glycerol phosphate in 1000 g of double-distilled water to obtain a genipin solution containing disodium glycerol phosphate. In the genipin solution containing disodium glycerol phosphate, The content of nipin is 1 wt%, and the content of disodium glycerol phosphate is 0.6 wt%.

[0032] Silk fibroin solution: 4g silk fibroin was dissolved in 100g double-distilled water to prepare a silk fibroin solution, and the silk fibroin content in the silk fibroin solution was 4wt%.

[0033] Among them, silk fibroin is prepared as follows:

[0034] Weigh 40-50g silk fiber into a container with a certain volume, add 2000ml of 0.5% sodium carbonate alkaline solution by mass concentration, heat and boil for 30min, wash twice with three distilled water, and then repeat the above steps twice, boil altogether Three ...

Embodiment 2

[0038] After taking 5 ml of the medicinal gelatin solution with a mass concentration of 3% prepared in Example 1 and injecting it into the submucosa of the isolated porcine small intestine through a syringe, 1.5 ml of the genipin solution prepared in Example 1 was injected into the above medicinal gelatin solution After 2 minutes, a semi-solid dark blue gel pad is formed to hold up the mucous membrane.

Embodiment 3

[0040] After taking 5 ml of the chitosan solution prepared in Example 1 into the submucosa of the isolated pig small intestine through a syringe, then injecting 2.5 ml of the genipin solution prepared in Example 1 into the above chitosan solution, which will form after 1 minute The semi-solid dark blue gel pad holds up the diseased mucosa.

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PUM

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Abstract

The invention relates to the field of biomedical engineering, and discloses an injectable colored gel pad for gastrointestinal mucosa delamination and application thereof. A fibroin solution, a chitosan solution or a gelatin solution is firstly injected into the submucosa of the digestive tract; and then, a genipin solution containing disodium glycerophosphate is injected into the submucosa of thedigestive tract; and a gel pad is formed in the submucosa of the digestive tract after a cross-linking reaction occurs, and is used for separating a pathological mucosal layer from a muscle layer tofacilitate the dissection of the pathological mucosal layer. The forming time of the gel pad is controlled by controlling the dosage of the genipin and the disodium glycerophosphate in the genipin solution. The injectable colored gel pad for gastrointestinal mucosa delamination has the advantages that used materials are all liquid materials with low cytotoxicity and high biocompatibility; the formed gel pad is dark blue, so that pathological tissues can be clearly marked, and the submucosal dissection operation is more convenient and safer; a good clinical use effect is achieved; and the injectable colored gel pad is worthy of clinical popularization.

Description

Technical field [0001] The invention relates to the field of biomedical engineering, and relates to an injectable colored gel pad used for the stratification of the digestive tract mucosa and its application. Background technique [0002] Early gastrointestinal cancer refers to cancer of the gastrointestinal tract located in the mucosa or submucosa, regardless of the size of the lesion and whether there is lymph node metastasis. Endoscopic mucosal resection (EMR) can perform endoscopic resection of early gastrointestinal cancer. The resection scope includes the mucosal layer, part of the submucosa and part of the normal mucosa around the cancer tissue. Endoscopic submucosal dissection (ESD) is developed on the basis of EMR technology. The current clinical treatment results show that ESD can improve the one-time complete resection rate and histologically curative resection rate. It is a kind of treatment for early gastrointestinal cancer. A safe and effective method has become on...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L31/04A61L31/14A61L31/18
CPCA61L31/047A61L31/042A61L31/045A61L31/145A61L31/14A61L31/18A61L2400/06C08L89/00C08L5/08
Inventor 杨宇民赵亚红王勇军张鲁中李贵才凌珏杨鹏翔
Owner NANTONG UNIVERSITY
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