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Foam control method in a kind of rhamnolipid fermentation process

A fermentation process and rhamnolipid technology, applied in the biological field, can solve problems such as affecting the fermentation efficiency, reducing the effective utilization volume of the fermenter, etc., achieving the effects of simple process operation, reducing the risk of contamination, and solving liquid escape

Active Publication Date: 2022-01-07
WANHUA CHEM GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Reducing the amount of liquid in the fermentation tank can alleviate the above problems to a certain extent, but it will reduce the effective use volume of the fermentation tank and affect the fermentation efficiency

Method used

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  • Foam control method in a kind of rhamnolipid fermentation process
  • Foam control method in a kind of rhamnolipid fermentation process

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] 1. Pick a single colony of Pseudomonas aeruginosa (Pseudomonas aeruginosa) KT1115 from the agar solid plate with an inoculation loop and inoculate it into the seed medium, culture at 30° C. and 200 rpm for 21 hours to obtain a seed solution.

[0038]The composition of the above-mentioned seed medium is as follows: peptone 10g / L, yeast powder 5g / L, NaCl 10g / L, and the balance is water.

[0039] 2. The seed liquid cultivated in step 1 is inoculated in the fermentation medium of a 7L fermenter (50% of filling capacity) by an inoculum size of 10% (volume ratio) to start fermentation and cultivation, and the initial OD600 after inoculation is 4.2, The fermentation conditions are: temperature 30° C., ventilation rate 1 vvm, stirring speed 300 rpm. During the fermentation process, the pH of the fermentation broth and the pressure of the fermenter are simultaneously adjusted at different stages, specifically: 0-6h, control the pH to 6.5, and the pressure of the fermenter to 0.0...

Embodiment 2

[0047] In addition to regulating the pH of the fermented liquid and the pressure of the fermenter simultaneously in different stages according to the following method during the fermentation process, repeat the steps of Example 1 to obtain the fermented liquid fermented for 10 days:

[0048] 0-6h, control the pH to 5, the pressure of the fermenter is 0.02MPa; 6-96h, control the pH to 6.5, the pressure of the fermenter is 0.04MPa; after 96h, control the pH to 5, the pressure of the fermenter is 0.015MPa.

[0049] After testing, the yield of rhamnolipid reached 0.52g / L / h.

Embodiment 3

[0051] In addition to regulating the pH of the fermented liquid and the pressure of the fermenter simultaneously in different stages according to the following method during the fermentation process, repeat the steps of Example 1 to obtain the fermented liquid fermented for 10 days:

[0052] 0-6h, control the pH to 8.5, the pressure of the fermenter is 0.035MPa; 6-96h, control the pH to 5.3, the pressure of the fermenter is 0.015MPa; after 96h, control the pH to 7.5, the pressure of the fermenter is 0.04MPa.

[0053] After testing, the yield of rhamnolipid reached 0.54g / L / h.

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Abstract

The invention discloses a foam control method in the rhamnolipid fermentation process. The foam control method in the rhamnolipid fermentation process disclosed by the invention comprises the following steps: fermenting and culturing Pseudomonas aeruginosa (Pseudomonas aeruginosa) in a fermentation medium, and controlling the pH of the fermented liquid at 5.0 in the early stage of fermentation 8.5, the pressure of the fermenter is controlled at 0.01-0.035MPa, the pH of the fermented liquid is controlled at 5.0-6.5 in the middle stage of fermentation, the pressure of the fermented tank is controlled at 0.015-0.05MPa, and the pH of the fermented liquid is controlled at 5.0-7.5 in the later stage of fermentation. The pressure is controlled at 0.015‑0.04MPa. The fermentation process provided by the invention can effectively solve the liquid escape problem caused by foaming in the rhamnolipid fermentation process, and at the same time, the post-separation process does not need to consider the removal of the defoaming agent. The fermentation process is simple to operate and is suitable for industrial production of rhamnolipids .

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a method for controlling foam in a rhamnolipid fermentation process. Background technique [0002] Rhamnolipid is a non-toxic, non-polluting, biodegradable, anionic surfactant with good selectivity and specificity, and has good surface activity. It is used in many industries such as medicine, food, cosmetics, and environmental protection. The field has unique application prospects. Although its application performance is good, because it is an anionic surfactant, aerobic fermentation needs to be carried out under aeration and stirring conditions, and a large amount of foam will be generated during the aeration and stirring fermentation process. Excessive foam will hinder the normal progress of fermentation An important factor; in addition, if the foam control method is improper, the foam overflows will bring out the bacterial cells and fermentation broth, which is easy to infect bacte...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/44C12Q3/00C12R1/385
CPCC12P19/44C12Q3/00
Inventor 王竞辉姜西娟杨付伟黎源黄真真孔令晓
Owner WANHUA CHEM GRP CO LTD
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