Production method of antibacterial peptide

Abstract

The invention discloses a production method of an antibacterial peptide. For a fermentation mash produced by fermentation of bacteria expressed in a form of fusion protein, an antibacterial peptide inthe fermentation mash can be effectively separated from other culture medium residues and thalli by a low-temperature standing and plate-frame filtering combined method, so that the antibacterial body can be purified, the titer of an obtained product is larger than or equal to 100,000 U / g, the yield is larger than or equal to 85%, and the cost is smaller than or equal to 35 Yuan / kg. Compared withantibacterial peptides produced by other methods on the market, the antibacterial peptide prepared by the production method has a greatly improved titer, an improved yield and a substantially reducedcost, breaks through the process bottleneck of mass production of the antibacterial peptide for feed by the method, and has better practicability.

Description

technical field [0001] The invention relates to the field of production of antibacterial peptides, in particular to a production method of antibacterial peptides. Background technique [0002] Antimicrobial peptides are a class of basic small molecule peptides with antibacterial activity, with a molecular weight of about 2000-7000. Most of these active small molecule peptides have broad-spectrum antibacterial characteristics, and are the best substitutes for banned antibiotics in the feed farming industry. [0003] Due to the early ban on antibiotics in feed in Europe and the United States, the research, development and application of antimicrobial peptides for feed are far ahead of China. Due to the protection of intellectual property rights and other issues, there have been almost no exchanges on antimicrobial peptide technology, whether international or domestic. At present, the antimicrobial peptides used as feed additives in the domestic market are all imported products...

AI Technical Summary

Problems solved by technology

Due to the killing effect of antimicrobial peptides on bacteria, prokaryotic expression systems cannot be used to directly express biologically active antimicrobial peptides, and if they are expressed in the form of fusion proteins, the expression products can hardly be separated and purified from residual culture medium and bacteria (no matter what method is used) separation equipment), the yield is low (about 25%), the product titer is low (about 30000u / g), and the cost is high (about 100 yuan / kg)