Major QTL locus of C07 chromosome for characters of main-inflorescence pod density in brassica napus, SNP molecular markers and application

Abstract

The invention provides a major QTL locus of C07 chromosome for characters of main-inflorescence pod density in brassica napus. The major QTL locus of the C07 chromosome for the characters of the main-inflorescence pod density in the brassica napus is located between a base at a 42696564th locus and a base at a 43484267th locus of the C07 chromosome of the brassica napus. Preferably, the contribution rate of the major QTL locus to the main-inflorescence pod density of the brassica napus is 17.51%. The major QTL locus is closely linked to a first SNP molecular marker which is a base G or T located at a 42696564th locus, and the mutation results in polymorphism. The major QTL locus is closely linked to a second SNP molecular marker which is a base C or T located at a 43484267th locus, the mutation results in polymorphism. The major QTL locus is closely linked to a peak-value SNP molecular marker which is a base G or T located at a 43199100th locus, and the mutation results in polymorphism. The invention further provides related SNP molecular markers as well as application. The major QTL locus of the C07 chromosome for the characters of the main-inflorescence pod density in the brassica napus has a high contribution rate to the characters of the main-inflorescence pod density in the brassica napus, and plays a key role in regulation and control over the main-inflorescence pod density in the brassica napus; and thus, the major QTL locus can be used for map position cloning and molecular marker assisted selection, and is suitable for large-scale application.

Description

technical field [0001] The invention relates to the technical field of molecular biology and rapeseed breeding, in particular to the technical field of silique density traits of the main inflorescence of Brassica napus, specifically refers to a C07 chromosome main effect QTL site and SNP of the silique density trait of the main inflorescence of Brassica napus Molecular markers and their applications. Background technique [0002] The demand for edible oil in my country is growing rigidly at a rate of 1 million tons per year. It is estimated that by 2025, the demand for vegetable oil will be 3.5-4.0 times that of the existing domestic production capacity. However, my country's arable land resources continue to decrease, so increasing the yield of oil crops The fundamental way to increase the total output of oilseeds. Rapeseed is the largest oil crop in my country, with an annual sowing area of ​​more than 100 million mu and a total output of more than 15 million tons. Rapesee...

AI Technical Summary

Problems solved by technology

Genes controlling seed density have been cloned in crops such as rice and corn, but little research has been done on silique density in rapeseed

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