Alpha-L-fucosidase assay kit

Abstract

The invention discloses an alpha-L-fucosidase assay kit, and relates to the technical field of biological detection. The alpha-L-fucosidase assay kit comprises a reagent R1 and a reagent R2 which areindependent of each other; and the alpha-L-fucosidase assay kit specifically comprises the following ingredients in the following contents: the reagent R1 consisting of 7.5-8.5 g / L of disodium hydrogen phosphate, 5.0-6.0 g / L of citric acid and 0.2-0.8 g / L of sodium azide, and the reagent R2 consisting of 7.5-8.5 g / L of disodium hydrogen phosphate, 5.0-6.0 g / L of citric acid, 0.2-0.8 g / L of sodiumazide, and 4-8 g / L of 2-chloro-4 nitrobenzene-alpha-L-fucopyranoside. In the process of implementation of the alpha-L-fucosidase assay kit, arginine, asparagine and sucrose are added into the reagentR1 and the reagent R2 as stabilizers, so that accuracy, precision and stability of the kit are significantly improved; and thus, an alpha-L-fucosidase assay kit with high accuracy, excellent precision, strong stability and good linear relationship is provided.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to an α-L-fucosidase assay kit. Background technique [0002] α-L-Fucosidase (α-L-Fucosidase, AFU) is a lysosomal acid hydrolase, which is widely distributed in various tissues, cells and body fluids in the human body. Tissues such as liver and kidney are more active, and their basic function is to catalyze the hydrolysis and metabolism of fucosyl-containing oligosaccharides, glycoproteins and glycosides. These aspects play an important role, studies have found that the determination of α-L-fucosidase has a positive role in the diagnosis and treatment of various diseases. [0003] The methods for determining α-L-fucosidase include fluorescence method, end-point colorimetric method and continuous monitoring method. The researchers of the fluorescence method use AFU to hydrolyze 4-methylumbelliferone α-L-fucopyranoside to release 4-methylumbelliferone, terminate the rea...

AI Technical Summary

Problems solved by technology

This method is easy to operate, short in measurement time, and has improved sensitivity and anti-interference to a certain extent, but it still cannot reach the ideal level, and there are problems of poor stability and low accuracy of the kit.

[0004] At present, there are very few kits for the determination of α-L-fucosidase that have been reported. Liu Jianwu "Determination of serum α-L-fucosidase by continuous monitoring method", "Journal of Clinical Laboratory", No. 03, 1997 A single reagent continuous monitoring method for serum α-L-fucosidase was developed. The reagents used were pH6.5 buffer solution, 0.1mol / L citric acid, 0.2mol / L disodium hydrogen phosphate, 1.0mmol / L p-nitrogen Phenol α-L-fucopyranoside, the detection result is that the measured values ​​of 20 serums were tested by paired t test, P>0.05, and the difference was not statistically significant; the same mixed serum was measured by this method, the intra-assay CV=5.0%, Batch-to-batch CV=7.4%; a high-value serum of 32U / L was diluted and measured, and still showed a good linear relationship r=0.999, bilirubin 115μmol / L, hemoglobin 32g / L and triglyceride 300μmol / L There is no obvious interference in the measured value, but the reagent has the disadvantages of instability and low accuracy

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