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Fruity-flavor yeast, its screening method and its application in cigarettes

A screening method, technology of fruit flavor type, applied in screening method and application in cigarettes, field of fruit flavor yeast, can solve environmental pollution and other problems, achieve the effect of improving smoking quality, strong fruity aroma, and improving aroma characteristics

Active Publication Date: 2022-04-08
TOBACCO RES INST CHIN AGRI SCI ACAD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The chemical method has an advantage in cost, but during the synthesis process, it will come into contact with substances that are more harmful to the human body and cause environmental pollution

Method used

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  • Fruity-flavor yeast, its screening method and its application in cigarettes
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  • Fruity-flavor yeast, its screening method and its application in cigarettes

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Embodiment Construction

[0025] 1. Materials and methods

[0026] 1.1 Materials, reagents and instruments

[0027] In July 2018, samples were collected from 19 locations in Lichuan City, Enshi Tujia Autonomous Prefecture, Hubei Province, at different altitudes in tobacco growing areas. The upper leaves, middle leaves, and lower leaves were sampled separately.

[0028] 1.2 Reagents and media

[0029] Test reagents: PCR reagents such as Taq enzyme, DNA molecular weight marker, DNA extraction kit and PCR primers were purchased from Qingdao Qingke Biotechnology Co., Ltd.; peptone (peptone), yeast powder (Yeast extract), glucose (Glucoseconcentration), chloride Sodium, tryptone, beef extract, ammonium sulfate and agar were purchased from Sinopharm Chemical Reagent Co., Ltd.; corn steep liquor (CSP), corn protein powder (Corn protein powder) and bean cake powder (Soybeancake powder) were purchased from Qingdao Branch Rui Culture Medium Company; chromatographic pure reagents such as phenylacetaldehyde, iso...

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Abstract

The invention discloses a fruity-flavor yeast, a screening method and an application in cigarettes. A screening method for fruity-flavor yeast according to the present invention comprises the following steps: (1) taking samples at multiple locations in tobacco planting areas at different altitudes; (2) weighing tobacco samples, cutting them into pieces and placing them in In the culture medium, vibrate culture, carry out gradient dilution of the culture solution, spread on the plate respectively, place in the incubator to cultivate, pick the single colony that grows and inoculate the plate; (3) step (2) cultured on the plate Single colonies were inoculated in liquid medium and cultured by shaking; (4) Sensory evaluation was carried out by smelling aroma, and the better the aroma quality, the better the fruity aroma yeast was selected. The aroma-producing fermentation liquid of the R59 strain was filtered and evenly sprayed on the surface of the shredded tobacco, and the comprehensive sensory evaluation was carried out after the cigarette was made. The fruity aroma of the fermented sample was stronger and fuller, the coordination of the aroma was also increased, and the aftertaste was cleaner, which could significantly improve the smoking quality .

Description

technical field [0001] The invention belongs to the field of microorganisms and fermentation, and in particular relates to a fruity-flavor yeast, a screening method and its application in cigarettes. Background technique [0002] The quality of flue-cured tobacco has an important impact on the development of the tobacco industry. Insufficient aroma of tobacco leaves has always been the main factor restricting the quality of tobacco leaves and its industrial usability. Especially with the development of coke reduction technology, the aroma in the flue gas will be further reduced, resulting in a decline in the quality of cigarettes. The acceptance of smokers is reduced, so higher requirements are placed on the aroma quality of tobacco leaves. At present, the measures to improve the aroma quality of tobacco leaves mainly focus on the measures of variety selection, improvement of cultivation techniques and addition of flavoring and ingredients in cigarette processing. In the in...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A24B3/12
CPCA24B3/12
Inventor 张鹏王致鹏樊俊师超申国明李金海高加明
Owner TOBACCO RES INST CHIN AGRI SCI ACAD
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