Fruity-flavor yeast, its screening method and its application in cigarettes
A screening method, technology of fruit flavor type, applied in screening method and application in cigarettes, field of fruit flavor yeast, can solve environmental pollution and other problems, achieve the effect of improving smoking quality, strong fruity aroma, and improving aroma characteristics
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[0025] 1. Materials and methods
[0026] 1.1 Materials, reagents and instruments
[0027] In July 2018, samples were collected from 19 locations in Lichuan City, Enshi Tujia Autonomous Prefecture, Hubei Province, at different altitudes in tobacco growing areas. The upper leaves, middle leaves, and lower leaves were sampled separately.
[0028] 1.2 Reagents and media
[0029] Test reagents: PCR reagents such as Taq enzyme, DNA molecular weight marker, DNA extraction kit and PCR primers were purchased from Qingdao Qingke Biotechnology Co., Ltd.; peptone (peptone), yeast powder (Yeast extract), glucose (Glucoseconcentration), chloride Sodium, tryptone, beef extract, ammonium sulfate and agar were purchased from Sinopharm Chemical Reagent Co., Ltd.; corn steep liquor (CSP), corn protein powder (Corn protein powder) and bean cake powder (Soybeancake powder) were purchased from Qingdao Branch Rui Culture Medium Company; chromatographic pure reagents such as phenylacetaldehyde, iso...
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