Super-resolution total internal reflection microscopic imaging device and method based on polarity scattering

Abstract

The invention discloses a super-resolution total internal reflection microscopic imaging device and method based on polarity scattering, and the device comprises a polarization illumination module, alight beam scanning module, and a polarization detection module, and the polarization illumination module, the light beam scanning module, and the polarization detection module are sequentially arranged in a light propagation direction. The polarization illumination module is sequentially provided with a laser, a first polaroid and a quarter-wave plate in the light propagation direction. The lightbeam scanning module is sequentially provided with a two-dimensional scanning galvanometer, a scanning lens, a first tube mirror, a conical reflector and a conical lens in the light propagation direction. The polarization detection module is sequentially provided with an objective lens, a second polaroid, a second tube mirror and a camera in the light propagation direction. According to the device and the method, super-resolution imaging can be realized without fluorescent dyeing, the dynamic bleaching characteristic of a sample without similar fluorescent imaging can be observed more truly,long-time imaging can be realized, super-resolution can be realized without marking, and m-time resolution improvement can be obtained according to different orders m.

Description

technical field [0001] The invention relates to the technical field of microscopic imaging, and more specifically relates to a super-resolution total internal reflection microscopic imaging device and method based on polar scattering. Background technique [0002] At present, in conventional super-resolution microscopy, fluorescent markers are usually required. Traditional fluorescence super-resolution microscopy techniques use the nonlinear effect of fluorescent particles to distinguish structures that are very close to achieve super-resolution effects. The super-resolution imaging method based on fluorescently labeled samples has strict requirements on both fluorescent particles and imaging samples, and is not universally applicable. Labeled samples cannot be imaged for a long time due to their photobleaching characteristics, and it is easy to cause rejection of organisms, affecting A theoretical study of the effects of motion on biological properties. [0003] In contras...

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Problems solved by technology

However, this method needs to overcome the fundamental physical bottleneck of the diffraction limit in optics, so the development is very slow, and the existing non-fluorescent label super-resolution microscopy technology has poor real-time performance and narrow field of view, and cannot solve the problem of fluorescence super-resolution microscopy well. Problems such as poor universality of microtechnology and short imaging time

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