Totally-enclosed inverted microscope with multi-region scanning function

Abstract

The invention discloses a totally-enclosed inverted microscope with a multi-region scanning function, which comprises an inverted microscope body, and the inverted microscope body comprises a light source, and the light source is connected with an optical lens assembly through a connecting structure; and the optical lens assembly is connected with a transverse moving structure and a longitudinal moving structure. A fixedly arranged transparent objective table is installed between the light source and the optical lens assembly. According to the invention, the light source and the optical lens assembly move synchronously, and the objective table is immovable, so that in-situ observation of a liquid culture is realized; the inverted microscope is positioned in the completely closed box body and is not influenced by a high-temperature and high-humidity environment in the incubator, so that the service life of the inverted microscope is prolonged; by means of the specially-arranged longitudinal moving mechanism, the occupied area of the microscope is greatly reduced; and the inverted microscope and the culture medium are isolated, so that the culture solution is effectively prevented from being scattered on the objective table to cause microscope pollution.

Description

technical field [0001] The invention discloses a fully enclosed inverted microscope with multi-region scanning functions, which relates to the field of microscopic technology. Background technique [0002] An inverted microscope is a microscope with an illumination system above the stage and an objective lens below the stage. It is usually used to observe cultured living cells. Generally speaking, living cells (such as microorganisms, animal cells, etc.) are cultured in containers (such as multi-well plates, petri dishes) filled with culture fluid, and the culture containers are placed in an incubator. When observing this type of cells, the culture container is generally taken out of the incubator and placed under an inverted microscope for observation. The following problems are prone to occur: 1. Since microorganisms or cells are in the culture solution, the cells are taken out of the incubator When taken out of the medium, it will cause the vibration of the culture mediu...

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Problems solved by technology

When observing this type of cells, the culture container is generally taken out of the incubator and placed under an inverted microscope for observation. The following problems are prone to occur: 1. Since microorganisms or cells are in the culture solution, the cells are taken out of the incubator When it is taken out, it will cause the vibration of the culture medium, making it difficult for microorganisms or cells to observe their natural movement and track it

2. Liquid flow easily interferes with cell growth, making it difficult to observe cells in situ

If the microscope is directly placed in the incubator for observation, it will lead to the following problems: 1. The incubator is a high-temperature and high-humidity environment, which is easy to corrode the inverted microscope and affect its service life, especially the lens of the microscope has higher requirements on optical performance. high, easily damaged

2. The liquid medium is easy to spill on the stage and contaminate the microscope

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