Preparation method of tannic acid standard sample
A standard sample, tannic acid technology, applied in the field of analysis and detection, to achieve the effect of good uniformity, good stability and accurate value
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0024] The preparation of embodiment 1 tannic acid standard sample
[0025] For the preparation process route of tannic acid standard sample, see figure 1 mentioned. Weigh 10.0g of tannic acid and place it in a 100mL beaker, add 50mL of 50% methanol formic acid solution (containing 1% volume concentration formic acid) in a water bath and heat at 40°C until fully dissolved. The glycan gel chromatography column was washed with 1L of 50% methanol solution (containing 1% formic acid) to remove impurities, and then eluted with 500 mL of 70% acetone, and the acetone eluted part was collected for 45 The acetone was evaporated under reduced pressure at ℃, and the aqueous phase was freeze-dried to obtain 3.24 g of off-white powdery solid tannic acid standard sample. The components of the standard sample of tannic acid were analyzed by high-performance liquid chromatography, and its chromatogram is shown in figure 2 . Its ultraviolet absorption wavelength scanning spectrum is shown...
Embodiment 2
[0026] Weigh 10.0 g of tannic acid and place it in a 100 mL beaker, add 50 mL of 50% methanol formic acid solution (containing 1% formic acid) in a water bath and heat at 40°C in a water bath until fully dissolved. The gel chromatographic column was first washed with 1L of 50% methanol solution (containing 1% formic acid) to remove impurities, and then eluted with 500mL of 70% acetone, and the acetone eluted part was collected and decomposed at 40°C. Acetone was removed by pressure evaporation, and the aqueous phase was freeze-dried to obtain 4.08 g of off-white powdery solid tannic acid standard sample.
Embodiment 3
[0027] The preparation of embodiment 3 tannic acid standard samples
[0028] Weigh 100.0g of tannic acid and place it in a 1000mL beaker, add 500mL volume percentage concentration of 50% methanol formic acid solution (containing 1% formic acid), heat it in a water bath at 40°C until fully dissolved, and put Sephadex LH-20 dextran on the reaction solution The gel chromatographic column was first rinsed with 10L of 50% methanol solution (containing 1% formic acid) to remove impurities, and then eluted with 5L of 70% acetone. The acetone was removed by pressure evaporation, and the aqueous phase was freeze-dried to obtain about 40 g of off-white powdery solid tannic acid standard samples.
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More