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A kind of riboflavin fermentation production method

A production method, riboflavin technology, applied in the field of fermentation engineering, can solve the problems of low purity, achieve low solubility, increase growth rate, and improve synthesis efficiency

Active Publication Date: 2021-07-20
HENAN JULONG BIOLOGICAL ENG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Third: Since riboflavin is easily decomposed into light flavin under alkaline conditions, and the higher the temperature, the easier it is to decompose, making the purity obtained in the post-fermentation process lower

Method used

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  • A kind of riboflavin fermentation production method
  • A kind of riboflavin fermentation production method
  • A kind of riboflavin fermentation production method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Pick a single colony of the starting strain of Bacillus subtilis RX21 and place it in 5 mL of seed liquid medium at a speed of 200 r / min at 37 °C for 12 h, transfer it to 300 mL of seed liquid medium at a speed of 200 r / min, Cultivate for 10 h at ℃ to obtain seed solution Z1.

Embodiment 2

[0048] Pick a single colony of the starting strain of Bacillus subtilis RX21 and place it in 5 mL of seed liquid medium at a speed of 200 r / min at 37 °C for 12 h, transfer it to 300 mL of seed liquid medium at a speed of 200 r / min, Cultivate at ℃ for 12 h to obtain seed solution Z2.

Embodiment 3

[0050] Pick a single colony of the starting strain of Bacillus subtilis RX21 and place it in 5 mL of seed liquid medium at a speed of 200 r / min at 37 °C for 12 h, transfer it to 300 mL of seed liquid medium at a speed of 200 r / min, Cultivate at ℃ for 14 h to obtain seed liquid Z3.

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Abstract

The invention relates to a riboflavin fermentation production method, which belongs to the technical field of fermentation engineering. In the method, uracil is added to the fermentation medium, and at the same time, a carbon source and a nitrogen source are added in batches during the fermentation process to obtain riboflavin-containing riboflavin. Crude fermented liquid; adjust the pH of the fermented liquid to 11-13 to fully dissolve the riboflavin, then acidify the fermented liquid to a pH of 5.0-6.0 to fully separate the riboflavin, add an oxidant, heat and stir, centrifuge, and collect the precipitate and drying to obtain crude riboflavin; adding an acid solution to the crude riboflavin for adjustment, adding an oxidizing agent to heat and stir to obtain riboflavin products. According to the method, the riboflavin product with high efficiency and high purity is obtained by optimizing the parameters and operations in the synthesis route and the extraction process.

Description

technical field [0001] The invention belongs to the technical field of fermentation engineering, and in particular relates to a riboflavin fermentation production method. Background technique [0002] Riboflavin (riboflavin) is a yellow, light-sensitive, slightly water-soluble solid, which is a condensation product of ribitol and 6,7-dimethylisoalloxazine. Based on this, it is named riboflavin. It exists in the form of flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD) in the cell, as a coenzyme of oxidoreductase, and as a hydrogen carrier in metabolic reactions. Most microorganisms and plants have the function of synthesizing riboflavin in vivo, while humans and animals lack riboflavin synthesis pathways and belong to riboflavin heterotrophs. Riboflavin is an essential growth factor in humans and animals, and riboflavin deficiency can occur in some cases. Therefore, the body needs to supplement the right amount of riboflavin. The human body needs about 0.3...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P25/00C12N1/20C12R1/125
CPCC12N1/20C12P25/00C12N1/205C12R2001/125
Inventor 马挺李静曹华杰刘小都李闯漫磊磊
Owner HENAN JULONG BIOLOGICAL ENG CO LTD
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