Method for improving fermentation efficiency of lysine

Abstract

The invention belongs to the technical field of amino acid fermentation and discloses a method for improving the fermentation efficiency of lysine. The method comprises the following steps: inoculating brevibacterium flavum for producing the lysine according to the inoculation quantity of 4 to 8 percent into a fermentation culture medium, wherein the fermentation temperature is 31 DEG C at 0 to 18hours and is 34 DEG C at the end of 18 hours, the ventilation ratio is 1:(0.6-0.8), the stirring rotating speed is 200 to 400 r / min and the fermentation total time is 50 to 60 hours; and in the fermentation process, feeding a glucose solution to maintain the residual sugar content to be 5 to 10 g / L, feeding an ammonium sulfate solution to maintain the ammonia nitrogen content to be 1 to 2 g / L, feeding ammonia water to control the pH to be 7.0 to 7.2 and feeding a defoaming agent to perform defoaming. By the method, the acid producing efficiency of the lysine and the sugar acid conversion rateare increased.

Description

technical field

[0001] The invention belongs to the technical field of amino acid fermentation, and in particular relates to a method for improving lysine fermentation efficiency. Background technique

[0002] Lysine, also known as L-lysine, lysine, etc., is one of the most important essential amino acids in human and animal nutrition. At present, the global production of lysine has reached more than 2.4 million tons, second only to glutamic acid , becoming the second largest amino acid variety in the world. The lysine slag is a new bacterial protein feed product produced by microbial fermentation using lysine waste liquid as a raw material. It belongs to the field of biological fermentation waste liquid treatment and is accompanied by the production of lysine. Lysine waste liquid contains a large amount of: bacterial protein, COD, BOD, reducing sugar, lysine, alanine, aspartic acid, leucine, etc., in addition, it also contains trace amounts of P, K, Ca, etc. Inorganic sal...

Examples

Embodiment 1

[0034] Utilize the method for preparing lysine fermentation medium with bacterial protein, it comprises the steps:

[0035] Take each raw material according to the following concentrations: bacterial protein distiller's grains joint hydrolyzate 200ml / L, glucose 40g / L, molasses 12g / L, ammonium sulfate 4g / L, sodium citrate 5g / L, potassium dihydrogen phosphate 10g / L, seven Magnesium sulfate water 0.8g / L, manganese sulfate tetrahydrate 0.02g / L, ferrous sulfate heptahydrate 0.02g / L, vitamin B 1 0.01g / L, biotin 0.5mg / L;

[0036]Add each raw material to water in turn, stir evenly, adjust the pH to 7.8 with 100g / L NaOH aqueous solution, and sterilize at 115°C for 10min to obtain the product.

[0037] The improvements of the fermentation medium of the present invention relative to the conventional medium mainly include:

[0038] Conventional medium: glucose 80g / L, corn steep liquor 10g / L, molasses 12g / L, ammonium sulfate 8g / L, potassium dihydrogen phosphate 10g / L, magnesium sulfate ...

Embodiment 2

[0049] Utilize the method for preparing lysine fermentation medium with bacterial protein, it comprises the steps:

[0050] Take each raw material according to the following concentrations: bacterial protein distiller's grains joint hydrolyzate 200ml / L, glucose 40g / L, molasses 12g / L, ammonium sulfate 4g / L, sodium citrate 5g / L, potassium dihydrogen phosphate 10g / L, seven Magnesium sulfate water 0.8g / L, manganese sulfate tetrahydrate 0.02g / L, ferrous sulfate heptahydrate 0.02g / L, vitamin B 1 0.01g / L, biotin 0.5mg / L;

[0051] Add each raw material to water in turn, stir evenly, adjust the pH to 7.8 with 100g / L NaOH aqueous solution, and sterilize at 115°C for 10min to obtain the product.

[0052] The preparation method of the cell protein distiller's grains joint hydrolyzate is as follows:

[0053] Grind and pulverize the bacterial protein, then add it to 8 times the weight of a 0.5M hydrochloric acid solution, stir evenly, then place it in a high-speed shearing machine at a s...

Embodiment 3

[0057] Utilize the technology of fermenting and producing lysine in fermentation medium, it comprises the steps:

[0058] The liquid volume in the 50L automatic fermentation tank is 30L fermentation medium, and the strain is Brevibacterium flavum XQ90; the Brevibacterium flavum is inserted into the fermentation medium according to the inoculation amount of 5%, the inoculation concentration is 2g / L, and the fermentation temperature is 0 -18h is 31°C, 18h-end is 34°C, the ventilation ratio is 1:0.7, the stirring speed is 300r / min, and the total fermentation time is 60h; during the fermentation process, the residual sugar content is maintained at 5 -10g / L, maintain ammonia nitrogen content at 1-2g / L by feeding 300g / L ammonium sulfate solution, feed ammonia water to control pH7.0-7.2, and feed defoamer to defoam.

technical field

[0001] The invention belongs to the technical field of amino acid fermentation, and in particular relates to a method for improving lysine fermentation efficiency. Background technique

[0002] Lysine, also known as L-lysine, lysine, etc., is one of the most important essential amino acids in human and animal nutrition. At present, the global production of lysine has reached more than 2.4 million tons, second only to glutamic acid , becoming the second largest amino acid variety in the world. The lysine slag is a new bacterial protein feed product produced by microbial fermentation using lysine waste liquid as a raw material. It belongs to the field of biological fermentation waste liquid treatment and is accompanied by the production of lysine. Lysine waste liquid contains a large amount of: bacterial protein, COD, BOD, reducing sugar, lysine, alanine, aspartic acid, leucine, etc., in addition, it also contains trace amounts of P, K, Ca, etc. Inorganic sal...

Examples

Embodiment 1

[0034] Utilize the method for preparing lysine fermentation medium with bacterial protein, it comprises the steps:

[0035] Take each raw material according to the following concentrations: bacterial protein distiller's grains joint hydrolyzate 200ml / L, glucose 40g / L, molasses 12g / L, ammonium sulfate 4g / L, sodium citrate 5g / L, potassium dihydrogen phosphate 10g / L, seven Magnesium sulfate water 0.8g / L, manganese sulfate tetrahydrate 0.02g / L, ferrous sulfate heptahydrate 0.02g / L, vitamin B 1 0.01g / L, biotin 0.5mg / L;

[0036]Add each raw material to water in turn, stir evenly, adjust the pH to 7.8 with 100g / L NaOH aqueous solution, and sterilize at 115°C for 10min to obtain the product.

[0037] The improvements of the fermentation medium of the present invention relative to the conventional medium mainly include:

[0038] Conventional medium: glucose 80g / L, corn steep liquor 10g / L, molasses 12g / L, ammonium sulfate 8g / L, potassium dihydrogen phosphate 10g / L, magnesium sulfate ...

Embodiment 2

[0049] Utilize the method for preparing lysine fermentation medium with bacterial protein, it comprises the steps:

[0050] Take each raw material according to the following concentrations: bacterial protein distiller's grains joint hydrolyzate 200ml / L, glucose 40g / L, molasses 12g / L, ammonium sulfate 4g / L, sodium citrate 5g / L, potassium dihydrogen phosphate 10g / L, seven Magnesium sulfate water 0.8g / L, manganese sulfate tetrahydrate 0.02g / L, ferrous sulfate heptahydrate 0.02g / L, vitamin B 1 0.01g / L, biotin 0.5mg / L;

[0051] Add each raw material to water in turn, stir evenly, adjust the pH to 7.8 with 100g / L NaOH aqueous solution, and sterilize at 115°C for 10min to obtain the product.

[0052] The preparation method of the cell protein distiller's grains joint hydrolyzate is as follows:

[0053] Grind and pulverize the bacterial protein, then add it to 8 times the weight of a 0.5M hydrochloric acid solution, stir evenly, then place it in a high-speed shearing machine at a s...

Embodiment 3

[0057] Utilize the technology of fermenting and producing lysine in fermentation medium, it comprises the steps:

[0058] The liquid volume in the 50L automatic fermentation tank is 30L fermentation medium, and the strain is Brevibacterium flavum XQ90; the Brevibacterium flavum is inserted into the fermentation medium according to the inoculation amount of 5%, the inoculation concentration is 2g / L, and the fermentation temperature is 0 -18h is 31°C, 18h-end is 34°C, the ventilation ratio is 1:0.7, the stirring speed is 300r / min, and the total fermentation time is 60h; during the fermentation process, the residual sugar content is maintained at 5 -10g / L, maintain ammonia nitrogen content at 1-2g / L by feeding 300g / L ammonium sulfate solution, feed ammonia water to control pH7.0-7.2, and feed defoamer to defoam.

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