Application of active polypeptides in preparing anti-coronavirus medicaments and medical foods
An active polypeptide, coronavirus technology, applied in the field of biomedicine, can solve problems such as adverse reactions and antibody failure, and achieve the effects of low cost and convenient manufacturing
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Embodiment 1
[0025] A kind of method of active peptide preparation is as follows:
[0026] 1. Put the seeds of legumes (such as soybeans, peas, etc.) in boiling water for 5 to 10 minutes, and then quickly cool them down to room temperature.
[0027] 2. Inoculate fermentation strains such as Bacillus subtilis according to a weight ratio of 1:1000, stir appropriately and place in a humid high-temperature (above 25°C) straw environment to ferment for 10-15 days to obtain fermentation materials (such as figure 1 as shown, figure 1 For soybean fermented material).
[0028] 3. Add an appropriate amount of water to the fermented material, pour it into a wall breaker, and then filter it with a 100-mesh sieve. At this time, the filtrate contains a variety of proteins and peptides.
[0029] 4. The filtrate is subjected to high-power ultrasonic crushing, and the protein and macromolecular peptides in the filtrate are broken into small molecular peptides.
[0030] 5. Filter the filtrate after ultra...
Embodiment 2
[0033] The 8 groups of 27 kinds of polypeptides obtained in Example 1 can all change the conformation of ACE2, so that ACE2 cannot bind to the virus. As for the docking of these small molecule peptides with ACE2 receptor molecules, the details are as follows:
[0034] ACE2 molecules are usually located on the luminal surface of epithelial cells, and when coronaviruses infect through the luminal surface of cells with ACE2, the infection efficiency is very high. Once the small molecule biologically active peptide binds to ACE2 through non-covalent bonds (hydrogen bonds, van der Waals attraction, hydrophobic or hydrophilic binding, and electrostatic attraction, etc.), the microenvironment of the cell surface changes, and the spatial conformation of ACE2 is affected by the small peptide. If changes occur, it will be difficult for coronaviruses such as SARS to interact with the extracellular domain of the ACE2 molecule and fail to mediate the entry of the virus into cells. Such as...
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