Preparation method and quality control method of high-purity polyphyllin VII

A high-purity technology of C. trichosanthesin is applied in the field of preparation of high-purity C. trichosanthesin VII, and achieves the effect of great practical significance and academic value.

Active Publication Date: 2020-06-30
GUANGXI INST OF CHINESE MEDICINE & PHARMA SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0020] The purpose of the present invention is to provide a kind of preparation method and quality control method of high-purity pagoda saponin VII, solve the problem of high-purity pagoda saponin VII chemical reference substance
The present invention carries out the rese

Method used

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  • Preparation method and quality control method of high-purity polyphyllin VII
  • Preparation method and quality control method of high-purity polyphyllin VII
  • Preparation method and quality control method of high-purity polyphyllin VII

Examples

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Effect test

Embodiment 1

[0091] A method for preparing high-purity saponin VII, characterized in that it comprises the following steps:

[0092] 1) Take 5 kg of dried husks of Chonglou yunnanensis, crush them, extract with ethanol under reflux at an extraction temperature of 75°C, filter the extract, combine the filtrates, recover ethanol, and obtain an extract;

[0093] 2) Suspend the extract with 1.2 times the volume of distilled water, then sequentially extract with petroleum ether, ethyl acetate, and n-butanol, let stand, combine the n-butanol layer, and concentrate to obtain the n-butanol extract;

[0094] 3) The n-butanol extract was chromatographed on a silica gel column and eluted with a chloroform-methanol system gradient, and the fractions containing papaya saponin VII were collected;

[0095] 4) Fractions were detected by thin-layer chromatography and then combined and concentrated to obtain crude crystals of papaya saponin VII with a purity of 80-90% by weight;

[0096] 5) Separating and ...

Embodiment 2

[0110] A method for preparing high-purity saponin VII, characterized in that it comprises the following steps:

[0111] 1) Take 3 kg of dried husks from Huazhonglou, crush them, and extract with ethanol under reflux at an extraction temperature of 78°C, filter the extract, combine the filtrates, recover ethanol, and obtain an extract;

[0112] 2) Suspend the extract with 1.3 times the volume of distilled water, then sequentially extract with petroleum ether, ethyl acetate, and n-butanol, let stand, combine the n-butanol layer, and concentrate to obtain the n-butanol extract;

[0113] 3) The n-butanol extract was chromatographed on a silica gel column and eluted with a chloroform-methanol system gradient, and the fractions containing papaya saponin VII were collected;

[0114] 4) Fractions were detected by thin-layer chromatography and then combined and concentrated to obtain crude crystals of papaya saponin VII with a purity of 80-90% by weight;

[0115] 5) Separating and pur...

Embodiment 3

[0129] A method for preparing high-purity saponin VII, characterized in that it comprises the following steps:

[0130] 1) Take 5 kg of dried husks of Chonglou yunnanensis, crush them, extract with ethanol under reflux at an extraction temperature of 80 °C, filter the extract, combine the filtrates, recover ethanol, and obtain an extract;

[0131] 2) Suspend the extract with 1 times the volume of distilled water, then sequentially extract with petroleum ether, ethyl acetate, and n-butanol, let stand, combine the n-butanol layer, and concentrate to obtain the n-butanol extract;

[0132] 3) The n-butanol extract was chromatographed on a silica gel column and eluted with a chloroform-methanol system gradient, and the fractions containing papaya saponin VII were collected;

[0133] 4) Fractions were detected by thin-layer chromatography and then combined and concentrated to obtain crude crystals of papaya saponin VII with a purity of 80-90% by weight;

[0134] 5) Separating and p...

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Abstract

The invention relates to a preparation method and a quality control method of high-purity polyphyllin VII. Dry shells of paris polyphylla smith var.yunnanensis (franch.)hand.-mazz, paris polyphylla smith var. chinensis(franch.)hara or paris polyphylla smith are used as raw materials, reflux extraction is carried out by an ethanol solution, and a batch extraction process of a polyphyllin VII chemical reference substance and an analytical determination method for purity, content and impurity inspection are established, so that the technical standard of the polyphyllin VII chemical reference substance is established, and a scientific basis and guarantee are provided for quality standard research of the polyphyllin VII chemical reference substance as a traditional Chinese medicine chemical reference substance and medicinal materials and preparations. The method is reasonable in process design, simple in process, high in separation speed and short in production period, the obtained productis high in purity, the content reaches 98% or above, the quality is controllable, and the method is suitable for industrial production and has good application prospects.

Description

technical field [0001] The invention relates to the technical field of compound preparation, in particular to a preparation method and quality control method of high-purity saponin VII. Background technique [0002] Chemical reference substances, also known as standard substances, are physical controls for research on quality standards of traditional Chinese medicine, quality testing and quality control. Research on chemical reference substances of traditional Chinese medicine is a very important part of standardization research of traditional Chinese medicine. In the quality control of pharmaceutical production, chemical reference substances of traditional Chinese medicine play an extremely important role, and are the basis and core of quality control of traditional Chinese medicine. [0003] Saponin VII is a saponin chemical component, which is one of the active components of plants such as Yunnan Chonglou, Huachonglou, Multi-leaf Chonglou, Aescin, and is also an index com...

Claims

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Application Information

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IPC IPC(8): C07J71/00G01N30/90G01N30/02
CPCC07J71/0005G01N30/90G01N30/02Y02A50/30
Inventor 柴玲刘布鸣饶伟文陈明生袁健童冯军黄艳
Owner GUANGXI INST OF CHINESE MEDICINE & PHARMA SCI
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