Nucleotide sequencing element, chip and sequencing analysis method
A nucleotide sequence and nucleotide technology, which is applied to the analysis of materials, biochemical equipment and methods, and material analysis through electromagnetic means, and can solve the problems of chip holes that cannot be completely cleared, noise, and noise increase.
Active Publication Date: 2022-05-24
连俊龙 +1
View PDF9 Cites 0 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
However, due to the charge (hydrogen ions) generated during the sequencing process, even if the wells of the chip are regularly washed with a buffer solution, they cannot be completely removed.
Due to the increase of noise caused by the accumulation of charge in the hole of the chip, the DNA sequence can only read about 200 base pairs (base pair, bp) in length
Furthermore, when the chip manufacturing process is shrinking technically, if there is residual charge in the chip hole, it will be more likely to be affected by noise.
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment Construction
[0081] In order to make the description of the present disclosure more detailed and complete, the embodiments and specific embodiments of the present disclosure will be described below with reference to the accompanying drawings; but this is not the only form of implementing or using the specific embodiments of the present disclosure. The embodiments disclosed below can be combined or substituted with each other under beneficial circumstances, and other embodiments can also be added to one embodiment without further description or description.
[0082] In addition, spatially relative terms, such as "lower", "upper", etc., are used to conveniently describe the relative relationship of one element or feature to other elements or features in the drawings. These spatially relative terms are intended to encompass different orientations of the device in use or operation in addition to the orientation shown in the figures. The device may be otherwise oriented (eg, rotated 90 degrees ...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
| Property | Measurement | Unit |
|---|---|---|
| height | aaaaa | aaaaa |
| height | aaaaa | aaaaa |
Login to View More
Abstract
A nucleotide sequencing element is provided, which includes a semiconductor substrate, a transistor, a dielectric layer, a ring electrode group and a conductor. A nucleotide sequencing chip is also provided, comprising the aforementioned nucleotide sequencing element and a sense amplifier. Another method for sequencing analysis is provided, which includes providing the aforementioned nucleotide sequencing chip, placing the nucleotide sequence fragment to be tested and the known nucleotide sequence fragment in the wells for polymerization and electrochemical reactions, respectively. , and analyze the electrical signal generated by the reaction to obtain the sequencing result.
Description
technical field [0001] The present invention relates to a sequencing element, a chip and a sequencing analysis method, and in particular, to a nucleotide sequencing element, a chip and a sequencing analysis method. Background technique [0002] Deoxyribonucleic acid (DNA) sequencing refers to the analysis of the base sequence of a specific DNA fragment, that is, the analysis of adenine (A), thymine (T), cytosine (C) and guanine (G). ) arrangement. DNA sequencing has been widely used, and the scope is roughly divided into six major projects: basic research (gene structure, gene function), gene body research, the origin of life, differences between human races or species, specific gene variants and diseases, and test reagents and new drug development. [0003] At present, DNA sequencing has been improved to next-generation sequencing, one of which is a sequencing method based on Ion Torrent, which uses integrated circuit chips to directly convert chemical signals into digita...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/28
CPCG01N27/4145G01N27/414G01N27/327C12Q1/6869C12Q2563/149
Inventor 连俊龙萧正辉
Owner 连俊龙